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IJAT Vol.8 No.1 pp. 95-101
doi: 10.20965/ijat.2014.p0095
(2014)

Paper:

Three-Dimensional Microassembly of Cell-Laden Microplates by in situ Gluing with Photocurable Hydrogels

Shotaro Yoshida, Koji Sato, and Shoji Takeuchi

Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan

Received:
August 2, 2013
Accepted:
November 26, 2013
Published:
January 5, 2014
Keywords:
three-dimensional, microassembly, cells, hydrogels, MEMS
Abstract

This paper describes a method for assembling cellladen microplates into three-dimensional (3D) microstructures by in situ gluing using photocurable hydrogels. We picked up cell-laden microplates with microtweezers, placed the plate perpendicular to one another on a microgroove device, and glued them by local photopolymerization of biocompatible Poly (Ethylene Glycol) (PEG) hydrogels. The advantage of this assembly method is its ability to construct 3D biological microstructures with targeted cells. We demonstrated the assembly of a 3D half-cube microstructure with genetically labeled cell-laden microplates. We believe our method is useful for engineering the positions of cells in 3D configurations for cell-cell interaction analysis and tissue engineering.

Cite this article as:
S. Yoshida, K. Sato, and S. Takeuchi, “Three-Dimensional Microassembly of Cell-Laden Microplates by in situ Gluing with Photocurable Hydrogels,” Int. J. Automation Technol., Vol.8, No.1, pp. 95-101, 2014.
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