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IJAT Vol.8 No.1 pp. 102-109
doi: 10.20965/ijat.2014.p0102
(2014)

Paper:

Improved Cytocompatibility of Nanosecond-Pulsed Laser-Treated Commercially Pure Ti Surfaces

Masayoshi Mizutani*1, Ryo Honda*2, Yuta Kurashina*2,
Jun Komotori*3, and Hitoshi Ohmori*4

*1Tohoku University, 6-6-01 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan

*2Graduate School, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan

*3Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan

*4RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan

Received:
August 7, 2013
Accepted:
December 11, 2013
Published:
January 5, 2014
Keywords:
implant, Titanium, laser treatment, surface modification, osteoblast-like cell
Abstract

In this study, we developed a surface modification technology for implants using commercially pure (cp) Ti. The technology used in this study leads to reduction in the time required for adhesion between bone and surfaces of implants. The existence ofmicroasperities and oxide layers is important to induce calcium phosphate precipitation and bone formation activity of osteoblasts. In addition, we focused on nanosecondpulsed laser treatment as a method to create both microasperities and oxide layers. First, we observed surface morphologies formed by laser treatment. An oxide layer with high oxygen concentration and microasperities on the order of 10 nm to 10 µm were produced. Moreover, the OH groups were created on the laser-treated surface. Second, by culturing osteoblasts on the laser-treated cp Ti surface, its effects on cell shape, proliferation, and activity of bone formation were evaluated. Even though cell proliferation was at a comparable level in these two surfaces, the ALP activity per cell number was improved by about four times in the laser-treated surface compared with that in the polished surface. On the laser-treated cp Ti surface, it was considered that the bone formation activity of osteoblasts was promoted without inhibiting cell proliferation. From the results of this study, it is possible to conclude that by treating cp Ti surfaces with a laser, a surface with good cytocompatibility can be created.

Cite this article as:
M. Mizutani, R. Honda, Y. Kurashina, <. Komotori, and H. Ohmori, “Improved Cytocompatibility of Nanosecond-Pulsed Laser-Treated Commercially Pure Ti Surfaces,” Int. J. Automation Technol., Vol.8, No.1, pp. 102-109, 2014.
Data files:
References
  1. [1] T. Hayakawa, K. Takahashi, M. Yoshinari, H. Okada, H. Yamamoto, M. Sato, and K. Nemoto, “Trabecular bone response to titanium implants provided with a thin carbonate-containing apatite coating applied using molecular precursor method,” Int J Oral Maxillofac Implants, Vol.21, Issue 6, pp. 851-858, 2006.
  2. [2] B. Setzer, M. Bächle, M. C. Metzger, and R. J. Kohal, “The geneexpression and phenotypic response of hFOB 1.19 osteoblasts to surface-modified titanium and zirconia,” Biomaterials, Vol.30, Issue 6, pp. 979-990, 2009.
  3. [3] G. Zhao, Z. Schwartz, M. Wieland, F. Rupp, J. Geis-Gerstorfer, D. L. Cochran, and B. D. Boyan, “High surface energy enhances cell response to titanium substrate microstructure,” J Biomed Mater Res A, Vol.74, Issue 1, pp. 49-58, 2005.
  4. [4] D. Khang, J. Lu, C. Yao, K. M. Haberstroh, and T. J. Webster, “The role of nanometer and sub-micron surface features on vascular and bone cell adhesion on titanium,” Biomaterials, Vol.29, Issue 8, pp. 970-983, 2008.
  5. [5] J. Lawrence, L. Hao, and H. R. Chew, “On the correlation between Nd:YAG laser-induced wettability characteristics modification and osteoblast cell bioactivity on a titanium alloy,” Surf Coat Technol, Vol.200, Issues 18-19, pp. 5581-5589, 2006.
  6. [6] L. Xie, X. Liao, G. Yin, Z. Huang, D. Yan, Y. Yao, W. Liu, X. Chen, and J. Gu, “Preparation, characterization, in vitro bioactivity, and osteoblast adhesion of multi-level porous titania layer on titanium by two-step anodization treatment,” J Biomed Mater, Vol.98, Issue 2, pp. 312-320, 2011.
  7. [7] L. Bren, L. English, J. Fogarty, R. Policoro, A. Zsidi, J. Vance, J. Drelich, N. Istephanous, and K. Rohly, “Hydrophilic/electronacceptor surface properties of metallic biomaterials and their effect on osteoblast cell activity,” J Adhes Sci Technol, 18, 15/16, 1711-1722, 2004.
  8. [8] Z. Schwartz, R. O. Navarrete, B. D. Boyan, M. Wieland, and D. L. Cochran, “Mechanisms regulating increased production of osteoprotegerin by osteoblasts cultured on microstructured titanium surfaces,” Biomaterials, Vol.30, Issue 20, pp. 3390-3396, 2009.
  9. [9] R. A. Gittens, T. McLachlan, R. Olivares-Navarrete, Y. Cai, S. Berner, R. Tannenbaum, Z. Schwartz, K. H. Sandhage, and B. D. Boyan, “The effects of combined micron-/submicron-scale surface roughness and nanoscale features on cell proliferation and differentiation,” Biomaterials, Vol.32, Issue 13, pp. 3395-3403, 2011.
  10. [10] T. Miyauchi, M. Yamada, A. Yamamoto, F. Iwasa, T. Suzawa, R. Kamijo, K. Baba, and T. Ogawa, “The enhanced characteristics of osteoblast adhesion to photofunctionalized nanoscale TiO2 layers on biomaterials surfaces,” Biomaterials, Vol.31, Issue 14, pp. 3827-3839, 2010.
  11. [11] T. Kokubo and H. Takadama, “How useful is SBF in predicting in vivo bone bioactivity?” Biomaterials, Vol.27, Issue 15, pp. 2907-2915, 2006.
  12. [12] T. M. Li, H. C. Huang, C. M. Su, T, Y. Ho, C. M. Wu, W. C. Chen, Y. C. Fong, and C. H. Tang, “Cistanche deserticola extract increases bone formation in osteoblasts,” J Pharm Pharmacol, Vol.64, Issue 6, pp. 897-907, 2012.
  13. [13] H. T. Chen, C. H. Hsiao, H. Y. Long, K. C. Chen, J. L. He, C. J. Chung, and C. H. Tang, “Micro-arc oxidation of β-titanium alloy: Structural characterization and osteoblast compatibility,” Surf Coat Technol, Vol.204, Issues 6-7, pp. 1126-1131, 2009.
  14. [14] C. Mangano, F. Mangano, A. De Rosa, V. Desiderio, R. D’Aquino, F. De Francesco, V. Tirino, G. Papaccio, and A. Piattelli, “The osteoblastic differentiation of dental pulp stem cells and bone formation on different titanium surface textures,” Biomaterials, Vol.31, Issue 13, pp. 3543-3551, 2010.
  15. [15] S. Nuernberger, C. Albrecht, V. Vecsei, S. Marlovits, H. Redl, and N. Cyran, “The influence of scaffold architecture on chondrocyte distribution and behavior in matrix-associated chondrocyte transplantation grafts,” Biomaterials, Vol.32, Issue 4, pp. 1032-1040, 2011.

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