Automated Construction System for 3D Lattice Structure Based on Alginate Gel Fiber Containing Living Cells
Kenichi Ohara*, Masaru Kojima**, Akira Fukushima**,
Shun Onozaki**, Mitsuhiro Horade**, Masumi Yamada***,
Minoru Seki***, Yasushi Mae**, and Tatsuo Arai**
*Department of Mechatronics Engineering, Faculty of Science and Technology, Meijo University, 1-501 Shiogamaguchi, Tenpaku, Nagoya, Aichi 468-8502, Japan
**Department of Systems Innovation, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan
***Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan
The construction of 3D tissue is an important issue in regenerative medicine. While small 3D tissue has been realized in vitro, large functional 3D tissue has not been achieved due to difficulties in supplying oxygen and nutrients to the tissue. In this paper, we propose automated 3D construction based on hydrogel fiber to produce active 3D tissues. Results of several preliminary experiments in generating suitable hydrogel fibers have resulted in a lattice structure as an example of 3D tissue. In experiments in 3D structure construction, we observed cell growth in the constructed lattice confirming structure functionality.
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