Automated Construction System for 3D Lattice Structure Based on Alginate Gel Fiber Containing Living Cells
Kenichi Ohara*, Masaru Kojima**, Akira Fukushima**,
Shun Onozaki**, Mitsuhiro Horade**, Masumi Yamada***,
Minoru Seki***, Yasushi Mae**, and Tatsuo Arai**
*Department of Mechatronics Engineering, Faculty of Science and Technology, Meijo University, 1-501 Shiogamaguchi, Tenpaku, Nagoya, Aichi 468-8502, Japan
**Department of Systems Innovation, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan
***Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan
The construction of 3D tissue is an important issue in regenerative medicine. While small 3D tissue has been realized in vitro, large functional 3D tissue has not been achieved due to difficulties in supplying oxygen and nutrients to the tissue. In this paper, we propose automated 3D construction based on hydrogel fiber to produce active 3D tissues. Results of several preliminary experiments in generating suitable hydrogel fibers have resulted in a lattice structure as an example of 3D tissue. In experiments in 3D structure construction, we observed cell growth in the constructed lattice confirming structure functionality.
Shun Onozaki, Mitsuhiro Horade, Masumi Yamada,
Minoru Seki, Yasushi Mae, and Tatsuo Arai, “Automated Construction System for 3D Lattice Structure Based on Alginate Gel Fiber Containing Living Cells,” J. Robot. Mechatron., Vol.25, No.4, pp. 665-672, 2013.
-  R. Langer et al., “Tissue Engineering,” Science, Vol.240, pp. 920-926, 1993.
-  L. G. Griffith et al., “Tissue Engineering – Current Challenges and Expanding Opportunities –,” Science, Vol.295, pp. 1009-1014, 2002.
-  Y. Haraguchi et al., “Concise Review: Cell Therapy and Tissue Engineering for Cardiovascular Disease,” Stem Cells Translational Medicine, Vol.1, pp. 136-141, 2012.
-  N. Asakawa et al., “Pre-vascularization of in vitro threedimensional tissues created by cell sheet engineering,” Biomaterials, Vol.31, pp. 3903-3909, 2009.
-  Y. Tsuda et al., “Cellular control of tissue architectures using a three-dimensional tissue fabrication technique,” Biomaterials, Vol.28, pp. 4939-4946, 2007.
-  K. Arai et al., “Three-dimensional inkjet biofabrication based on designed images,” Biofabrications, Vol.3, doi:10.1088/1758-5082/3/3/034113, 2011.
-  C. Norotte et al., “Scaffold-free vascular tissue engineering using bioprinting,” Biomaterials, Vol.30, pp. 5910-5917, 2009.
-  K. Jakob et al., “Tissue engineering by self-assembly and bioprinting of living cells,” Biofabrication, Vol.2, doi:10.1088/1758-5082/2/2/022001, 2010.
-  J. S. Miller et al., “Rapid casting of patterned vascular networks for perusable engineered three-dimensional tissues,” Nature materials, Vol.11, pp. 768-774, 2012.
-  M. Yamada et al., “Microfluidic synthesis of chemically and physically anisotropic hydrogel microfibers for guided cell growth and networking,” Soft Matter, Vol.8, pp. 3122-3130, 2012.
-  M. Yamada et al., “Controlled formation of heterotypic hepatic micro-organoids in anisotropic hydrogel microfibers for longterm preservation of liver specific functions,” Biomaterials, Vol.33, pp. 8304-8315, 2012.
-  M. Iwase et al., “Fabrication of Vascular Tissue Models by Assembling Multiple Cell Types inside Hydrogel Microchannels,” Proc. of 2012 Int. Symposium on Micro-NanoMechatronics and Human Science, pp. 402-405, 2012.
-  H. Onoe et al., “Core-Shell Gel Wires for the Construction of Large Area Heterogeneous Structures with Biomaterials,” Proc. of 2010 IEEE 23th Int. Conf. on Micro Electro Mechanical Systems, pp. 248-251, 2010.
-  H. Onoe et al., “Living Cell Fabric,” Proc. of 2011 IEEE 24th Int. Conf. on Micro Electro Mechanical Systems, pp. 908-911, 2011.
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