Design and Fabrication of Changeable Cell Culture Mold
Puwanan Chumtong*, Masaru Kojima*, Kenichi Ohara**,
Yasushi Mae*, Mitsuhiro Horade*, Yoshikatsu Akiyama***,
Masayuki Yamato***, and Tatsuo Arai*
*Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan
**Faculty of Science and Technology, Meijo University, 1-501 Shiogamaguchi, Tempaku, Nagoya, Aichi 468-8502, Japan
***Institute of Advanced Biomedical Engineering and Science (ABMES) at TWIns, Tokyo Women’s Medical University (TWMU), 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan
-  T. Shimizu, M. Yamato, A. Kikuchi, and T. Okano, “Cell sheet engineering for myocardial tissue reconstruction,” Biomaterials, Vol.24, No.13, pp. 2309-2316, 2003.
-  Y. Haraguchi, T. Shimizu, T. Sasagawa, H. Sekine, K. Sakaguchi, T. Kikuchi, W. Sekine, S. Sekiya, M. Yamato, M. Umezu, and T. Okano, “Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro,” Nature Protocols, Vol.7, No.5, pp. 850-858, 2012.
-  K. Sugibayashi, Y. Kumashiro, T. Shimizu, J. Kobayashi, and T. Okano, “A molded hyaluronic acid gel as a micro-template for blood capillaries,” J. of Biomaterials Science, Polymer Edition, Vol.24, 2013.
-  N. N. Kachouie, Y. Du, H. Bae, M. Khabiry, A. F. Ahari, B. Zamanian, J. Fukuda, and A. Khademhosseini, “Directed assembly of cell-laden hydrogels for engineering functional tissues,” Organogenesis, Vol.6, No.4, pp. 234-244, 2011.
-  A. Nishiguchi, H. Yoshida, M. Matsusaki, and M. Akashi, “Rapid construction of three-dimensional multilayered tissues with endothelial tube networks by the cell-accumulation technique,” Advanced Materials, Vol.23, pp. 3506-3510, 2011.
-  V. L. Tsang and S. N. Bhatia, “Three-dimensional tissue fabrication,” Advanced Drug Delivery Reviews, pp. 1635-1647, 2004.
-  T. Masuda, N. Takei, T. Nakano, T. Anada, O. Suzuki, and F. Arai, “A microfabricated platform to form three-dimensional toroidal multicellular aggregate,” Biomedical Microdevices, Vol.14, Issue 6, pp. 1085-1093, 2012.
-  T. Anada, T. Masuda, Y. Honda, J. Fukuda, F. Arai, T. Fukuda, and O. Suzuki, “Three-dimensional cell culture device utilizing thin membrane deformation by decompression,” Sensors and Actuators B: Chemical, Vol.147, pp. 376-379, 2010.
-  T. A. Gwyther, J. Z. Hu, K. L. Billiar, and M. W. Rolle, “Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering,” J. of Visualized Experiments, Issue 57, 2011.
-  B. Guillotin and F. Guillemot, “Cell patterning technologies for organotypic tissue fabrication,” Trends in Biotechnology, Vol.29, No.4, pp. 183-190, 2011.
-  M. de Volder and D. Reynaerts, “Pneumatic and hydraulic microactuators: a review,” J. of Micromechanics and Microengineering, Vol.20, No.4, 2010.
-  J. H. Ryoo, G. S. Jeong, E. Kang, and S. H. Lee, “Ultrathin, hyperelastic PDMS nano membrane: fabrication and characterization,” Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences, Seattle, Washington, USA, pp. 686-688, 2011.
-  T. Anada, J. Fukuda, Y. Sai, and O. Suzuki, “An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids,” Biomaterials, Vol.33, Issue 33, pp. 8430-8441, 2012.
-  T. C. Merkel, V. I. Bondar, K. Nagai, B. D. Freeman, and I. Pinnau, “Gas Sorption, Diffusion, and Permeation in Poly(dimethylsiloxane),” J. of Polymer Science: Part B, Vol.38, pp. 415-434, 2000.
-  M. Johnson, G. Liddiard, M. Eddings, and B. Gale, “Bubble inclusion and removal using PDMS membrane-based gas permeation for applications in pumping, valving and mixing in microfluidic devices,” J. of Micromechanics and Microengineering, Vol.19, No.9, 2009.
-  K. Khanafer, A. Duprey, M. Schlicht, and R. Berguer, “Effects of strain rate, mixing ratio, and stress-strain definition on the mechanical behavior of the polydimethylsiloxane (PDMS) material as related to its biological applications,” Biomedical Microdevices, Vol.11, Issue 2, pp. 503-508, 2009.
-  S. Sang and H. Witte, “Fabrication of a surface stress-based PDMS micro-membrane biosensor,” Microsystem Technologies, Vol.16, Issue 6, pp. 1001-1008, 2010.
-  A. L. Thangawng, R. S. Ruoff, M. A. Swartz, and M. R. Glucksberg, “An ultra-thin PDMS membrane as a bio/micro-nano interface: fabrication and characterization,” Biomedical Microdevices, Vol.9, Issue 4, pp. 587-595, 2007.
-  H. Wu, B. Huang, and R. N. Zare, “Construction of microfluidic chips using polydimethylsiloxane for adhesive bonding,” The Royal Society of Chemistry, Vol.5, pp. 1393-1398, 2005.
-  B.-H. Jo, L. M. van Lerberghe, K. M. Motsegood, and D. J. Beebe, “Three-Dimensional Micro-Channel Fabrication in Polydimethylsiloxane (PDMS) Elastomer,” J. of Microelectromechanical Systems, Vol.9, No.1, pp. 76-81, 2000.
-  Y. Zhang, M. Ishida, Y. Kazoe, Y. Sato, and N. Miki, “Water-Vapor Permeability Control of PDMS by the Dispersion of Collagen Powder,” Trans. on Electrical and Electronic Engineering, Vol.4, No.3, pp. 442-449, 2009.
Supporting Online Materials:[a] “National data reports of the Organ Procurement and Transplantation Network (OPTN),” Health Resources and Services Administration, U.S. Department of Health and Human Services.
http://optn.transplant.hrsa.gov/ [Accessed January 18, 2013]
This article is published under a Creative Commons Attribution-NoDerivatives 4.0 Internationa License.
Copyright© 2013 by Fuji Technology Press Ltd. and Japan Society of Mechanical Engineers. All right reserved.