Optical Odor Imaging by Fluorescence Probes
Hirotaka Matsuo*, Yudai Furusawa*, Masashi Imanishi**,
Seiichi Uchida***, and Kenshi Hayashi*
*Department of Electronics, Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
**Graduate School of Systems Life Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
***Department of Advanced Information Technology, Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
Odor gas detection is important for the detection of explosives, environmental sensing, biometrics, foodstuffs and a comfortable life. Such odor-source localizations is an active research area for robotics. In this study, we tried to detect odor chemicals with an optical method that can be applied for the spatiotemporal detection of odor. We used four types of fluorescence dyes; tryptophan, quinine sulfate, acridine orange, and 1-anilinonaphthalene-8-sulfonate (ANS). As analyses, we measured the following four odor chemicals, 2-furaldehyde, vanillin, acetophenone, and benzaldehyde. The fluorescence-quenching mechanism of PET (Photoinduced Electron Transfer) or FRET (Fluorescence Resonance Electron Transfer), which occur between fluorescence dyes and odor compounds, could prevent unintended detection of various odorants that is caused by their unspecific adsorption onto the detecting materials. The fluorescence changes were then observed. Thus, we could detect the odor substances through fluorescent quenching by using the fluorescence dyes. Odor information could be obtained by response patterns across all the fluorescence dyes. Moreover, we captured odor images with a cooled CCD camera. Shapes of the targets that emitted odor could be roughly recognized by the odor-shape images. From the spatiotemporal images of odors, twodimensional odor expanse could be obtained.
-  T. Kikas, H. Ishida, D. R. Webster, and J. Janata, “Chemical Plume Tracking. 1. Chemical Information Encoding,” Anal. Chem., Vol.73, pp. 3662-3668, 2001.
-  G. Kowadlo and R. A. Russell, “Robot Odor Localization: a Taxonomy and survey,” Int. J. Robotics Res., Vol.27, pp. 869-894, 2008.
-  T. Nakamoto and H. Ishida, “Chemical Sensing in Spatial/Temporal Domains,” Chem. Rev., Vol.108, pp. 680-704, 2008.
-  J.-G. Li, Q.-H. Meng, Y. Wang, and M. Zeng, “Odor Source Localization using a Mobile Robot in Outdoor Airflow Environments with a particle Filter Algorithm,” Auton Robot, Vol.30, pp. 281-292, 2011.
-  G. Kowadlo and R. A. Russel, “Improving the Robustness of Naïve Physics Airflow Mapping, using Bayesian Reasoning on a Multiple Hypothesis Tree,” Robotics Auton. Sys., Vol.57, pp. 723-737, 2009.
-  A. loutfi, S. Coradeschi, A. Lilienthal, and J. Gonzalez, “Gas Distribution Mapping of Multiple Odor Sources using Mobile Robot,” Vol.27, pp. 311-319, 2008.
-  J. W. Gardner and P. N. Bartlett, “Electronic Noses: Principles and Applications,” Oxford University Press, 1999.
-  F. Rock, N. Barsan, and U. Weimar, “Electronic Nose: Current Status and Future Trends,” Chem. Rev., Vol.108, pp. 705-725, 2008.
-  Y. Hotokebuchi, K. Hayashi, K. Toko, R. Chen, and H. Ikezaki, “Fabrication of Odor Sensor using Peptide,” Vol.130, pp. 282-287, 2010.
-  B. Malnic, J. Hirono, T. Sato, and L. B. Buck, “Combinatorial Receptor Codes for Odors,” Cell, Vol.96, pp. 713-723, 1999.
-  K. Hayashi, “Development of odor code sensor recognizing substructure of odor molecules,” Proc. 25th Sensor Symp., pp. 607-610, 2008.
-  H. Nanto et al., “Novel gas sensor using polymer-film-coated quarts resonator for environmental monitoring,” Material Science and Engineering, Vol.C12, pp. 43-48, 2000.
-  Y. Sasaki, K. Hayashi, and K. Toko, “Fabrication of Odor Sensor Surface Recognizing Substructure of Odorant,” Sensor and Materials, Vol.21, pp. 191-199, 2009.
-  H. Matsuo and K. Hayashi, “Detection of Odor Map Image using Optical Method,” Proc. Int. Conf. Adv. Mech., Vol.5, pp. 165-170, 2010.
-  K. J. Albert, D. R.Walt, D. S. Gill, and T. C. Pearce, “Optical Multibead Arrays for Simple and Complex Odor Discrimination,” Anal. Chem., Vol.73, pp. 2501-2508, 2001.
-  M. Torimura et al., “Fluorescence-Quenching Phenomenon by Photoinduced Electron Transfer between a Fluorescent Dye and a Nucleotide Base,” Analytical Sciences, Vol.17, pp. 155-160, 2001.
-  Q. Chen et al., “Studies on fluorescence resonance energy transfer between dyes and water-soluble quantum dots,” Luminescence, Vol.20, pp. 251-255, 2005.
-  M. Imahashi, S. Nakayama, K. Miyagi, T. Takamizawa, and K. Hayashi, “Odor mapping using odor separating system,” Proc. 27th Sens. Symp., pp. 395-400, 2010.
Copyright© 2012 by Fuji Technology Press Ltd. and Japan Society of Mechanical Engineers. All right reserved.