2DOF Magnetically Driven Microtool for Soft Peeling of Zona Pellucida
Yoko Yamanishi*, Tomohiro Kawahara**, Tomohiro Iyanagi***,
Masaya Hagiwara**, Takehito Mizunuma***, Naoki Inomata**,
Shogo Kudo**, and Fumihito Arai***
*JST PRESTO, Department of Mechanical Science & Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
**Nagoya University, Japan
***Tohoku University, 6-6-01 Aramaki-Aoba, Aoba-ku, Sendai 980-8579, Japan
We automated removal of the swine oocyte zona pellucida using a 2DOF magnetically driven microtool (MMT) on a microfluidic chip. Multiple oocytes can be manipulated simultaneously highly stably, providing strong advantages over conventional manual manipulation using pipetting. We propose automating this process by activating a soft polymer microtool on a disposable microfluidic chip to stably mass-produce peeled oocytes – a breakthrough in high throughput and effective oocyte manipulation in cloning and fertility treatment.
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