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JRM Vol.22 No.5 pp. 594-600
doi: 10.20965/jrm.2010.p0594
(2010)

Paper:

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Microfluidic Device with Integrated Glucose Sensor for Cell-Based Assay in Toxicology

Hiroshi Kimura*, Hirokazu Takeyama*, Kikuo Komori*,
Takatoki Yamamoto**, Yasuyuki Sakai*, and Teruo Fujii*

*Institute of Industrial Science, The University of Tokyo, Tokyo 153-8505, Japan

**Department of Mechanical and Control Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550, Japan

Received:
February 26, 2010
Accepted:
June 8, 2010
Published:
October 20, 2010
Creative Commons License
Keywords:
cell-based assay, microfluidic device, electrochemical sensor, glucose, Hep G2 cell
Abstract
We have developed a cell-based assay platform using a microfluidic device integrating a glucose sensor into a cell culture device with closed-loop perfusion. Online measurement of cell kinetic change associated with cell status change was achieved by measuring glucose concentration change in the device with a cell exposed to a toxic material. The cell-based assay platform, which is integrated with a sensor and a perfusion system, was expected to improve measurement accuracy and efficiency, leading to the discovery of new tools in such wide-ranging fields as drug discovery, life sciences, and medical research.
Cite this article as:
H. Kimura, H. Takeyama, K. Komori, T. Yamamoto, Y. Sakai, and T. Fujii, “Microfluidic Device with Integrated Glucose Sensor for Cell-Based Assay in Toxicology,” J. Robot. Mechatron., Vol.22 No.5, pp. 594-600, 2010.
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