Microfluidic Device with Integrated Glucose Sensor for Cell-Based Assay in Toxicology
Hiroshi Kimura*, Hirokazu Takeyama*, Kikuo Komori*,
Takatoki Yamamoto**, Yasuyuki Sakai*, and Teruo Fujii*
*Institute of Industrial Science, The University of Tokyo, Tokyo 153-8505, Japan
**Department of Mechanical and Control Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550, Japan
We have developed a cell-based assay platform using a microfluidic device integrating a glucose sensor into a cell culture device with closed-loop perfusion. Online measurement of cell kinetic change associated with cell status change was achieved by measuring glucose concentration change in the device with a cell exposed to a toxic material. The cell-based assay platform, which is integrated with a sensor and a perfusion system, was expected to improve measurement accuracy and efficiency, leading to the discovery of new tools in such wide-ranging fields as drug discovery, life sciences, and medical research.
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