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JRM Vol.22 No.5 pp. 587-593
doi: 10.20965/jrm.2010.p0587
(2010)

Paper:

Parallel Formation of Three-Dimensional Spheroid Using Microrotational Flow

Hiroki Ota and Norihisa Miki

Department of Mechanical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohoama 223-8522, Japan

Received:
February 19, 2010
Accepted:
June 8, 2010
Published:
October 20, 2010
Keywords:
lab-on-a-chip, spheroid, microrotation flow, hepatocyte
Abstract

We propose three-dimensional (3D) spheroid formation involving perfusion and a lab-on-a-chip containing spheroid-forming chamber arrays. Cells are collected forming a spheroid in the chamber in microrotation. We previously reported a single chamber form hepatic spheroids 130 to 430 µm in diameter, controlling size by varying chamber diameter and cell density. Here, we scaled the system up by a factor of 10 while maintaining size control of 180±30 µm in diameter. Results were comparable to those using a single-chamber device. Long-term culture confirmed that cells in the spheroid maintained viability and diameters did not change after 24 hours. The system is readily applicable for creating size-controlled spheroids ensuring reliable, predictable in vitro data for drug screening and biological research.

Cite this article as:
Hiroki Ota and Norihisa Miki, “Parallel Formation of Three-Dimensional Spheroid Using Microrotational Flow,” J. Robot. Mechatron., Vol.22, No.5, pp. 587-593, 2010.
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