Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation
Yoko Yamanishi*1, Shinya Sakuma*2, Tomohiro Iyanagi*2,
Fumihito Arai*3, Tatsuo Arai*4, Akiyuki Hasegawa*4,
Tamio Tanikawa*5, Akihiko Ichikawa*5, Osamu Satoh*6,
Akihiro Nakayama*6, Hiroshi Aso*7, Mitsuhiro Goto*7,
Seiya Takahashi98, and Kazutsugu Matsukawa*8
*1JST PRESTO, Department of Mechanical Science & Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
*2Tohoku University, Japan
*3Nagoya University, Japan
*4Osaka University, Japan
We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and (4) they are fused by an electrical field. For the current study, the all-in-one unified microfluidic chip was designed to execute (1) cutting, (2) sorting, and (3) coupling parts continuously for this process. Basic functions of these parts as well as fusion part are verified independently. Then, all-in-one unified microfluidic chip was successfully designed and fabricated.
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