Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation

Yoko Yamanishi; Shinya Sakuma; Tomohiro Iyanagi; Fumihito Arai; Tatsuo Arai; Akiyuki Hasegawa; Tamio Tanikawa; Akihiko Ichikawa; Osamu Satoh; Akihiro Nakayama; Hiroshi Aso; Mitsuhiro Goto; Seiya Takahashi; Kazutsugu Matsukawa

doi:10.20965/jrm.2010.p0371

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JRM Vol.22 No.3 pp. 371-379

doi: 10.20965/jrm.2010.p0371

(2010)

Paper:

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Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation

Yoko Yamanishi^1, Shinya Sakuma^2, Tomohiro Iyanagi^2,
Fumihito Arai^3, Tatsuo Arai^4, Akiyuki Hasegawa^4,
Tamio Tanikawa^5, Akihiko Ichikawa^5, Osamu Satoh^6,
Akihiro Nakayama^6, Hiroshi Aso^7, Mitsuhiro Goto^7,
Seiya Takahashi⁹⁸, and Kazutsugu Matsukawa^*8

^*1JST PRESTO, Department of Mechanical Science & Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan

^*2Tohoku University, Japan

^*3Nagoya University, Japan

^*4Osaka University, Japan

^*5AIST, Japan

^*6KHI, Japan

^*7FHK, Japan

^*8NILGS, Japan

Received:

November 16, 2009

Accepted:

March 12, 2010

Published:

June 20, 2010

Keywords:

cell manipulation, microTAS, biochip, photolithography, cloning

Abstract

We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and (4) they are fused by an electrical field. For the current study, the all-in-one unified microfluidic chip was designed to execute (1) cutting, (2) sorting, and (3) coupling parts continuously for this process. Basic functions of these parts as well as fusion part are verified independently. Then, all-in-one unified microfluidic chip was successfully designed and fabricated.

Cite this article as:

Y. Yamanishi, S. Sakuma, T. Iyanagi, F. Arai, T. Arai, A. Hasegawa, T. Tanikawa, A. Ichikawa, O. Satoh, A. Nakayama, H. Aso, M. Goto, S. Takahashi, and K. Matsukawa, “Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation,” J. Robot. Mechatron., Vol.22 No.3, pp. 371-379, 2010.

Data files:

References

[1] K. H. Campbell, P. Loi, P. Cappai, and I. Wilmut, “Improved Development to Blastocyst of Ovine Nuclear Transfer Embryos Reconstructed during the Presumptive S-Phase of Enucleated Activated Oocytes,” Biology of Reproduction Vol.50, pp. 1385-1393, 1994.
[2] Y. Kimura and R. Yanagimachi, “Intracytoplasmic sperm injection in the mouse,” Biology of Reproduction, Vol.52, No.4, p. 709, 1995.
[3] T. Arai, “Automated Embryonic Cell Manipulation Using Micro Robotics Technology,” IEEE Int. Conf. on Intelligent Robotics and Systems (IROS), in Workshop “Bio-medical Micro-Nano Robotics: New trends and future aspects in the next decade,” 2007.
[4] A. Hasegaw, H. Uvet, K. Ohara, T. Takubo, Y. Mae, and T. Arai, “Self-controlled cell supply system for somatic cell cloning work in microfluidic chip,” MicroTAS2009, pp. 1255-1257, 2009.
[5] A. Ichikawa, T. Tanikawa, K. Matsukawa, S. Takahashi, and K. Ohba, “Fluorescent Monitoring Using Microfludics Chip and Development of Syringe Pump for Automation of Enucleation to Automate Cloning,” IEEE Int. Conf. on Robotics and Automation (ICRA), pp. 2231-2236, 2009.
[6] S. Sakuma, Y. Yamanishi, and F. Arai, “Magnetically Driven Microtools Actuated by a Focused Magnetic Field for Separating of Micro-particles,” J. of Robotics and Mechatronics, Vol.21, No.2, pp. 209-215, 2009.
[7] Y. Yamanishi, S. Sakuma, K. Onda, and F. Arai, “Biocompatible Polymeric Magnetically Driven Microtool for Particle Sorting,” J. of Micro and Nano Mechatronics, Vol.4, No.1, pp. 49-57, 2008.
[8] O. Satoh, A. Nakayama, S. Yamada, F. Arai, Y. Yamanishi, and S. Sakuma, “Investigation of On-chip Cell Coupling Module in Microfluidic Environments,” System Integration (SI2008), pp. 593-594, 2008.
[9] H. Aso, M. Goto, S. Yamada, F. Arai, and S. Sakuma, “The cell fusion module using minute channel environment,” System Integration (SI2008), pp. 595-596, 2008.
[10] U. Zimmerman and J. Vienken, “Electric field-induced cell-to-cell fusion,” J. Membrane Biol., Vol.67, pp. 165-182, 1982.
[11] M. Yamada, K. Kano, Y. Tsuda, J. Kobayashi, M. Yamato, M. Seki, and T. Okano, “Microfluidic devices for size-dependent separation of liver cells,” Biomedical Microdevices, Vol.9, pp. 637-645, 2007.
[12] M. Yamada, J. Kobayashi, M. Yamato, M. Seki, and T. Okano, “Millisecond treatment of cells using microfluidic devices via twostep carrier-medium exchange,” Lab on a Chip, Vol.8, pp. 772-778, 2008.

This article is published under a Creative Commons Attribution-NoDerivatives 4.0 Internationa License.

[1] [1] K. H. Campbell, P. Loi, P. Cappai, and I. Wilmut, “Improved Development to Blastocyst of Ovine Nuclear Transfer Embryos Reconstructed during the Presumptive S-Phase of Enucleated Activated Oocytes,” Biology of Reproduction Vol.50, pp. 1385-1393, 1994.

[2] [2] Y. Kimura and R. Yanagimachi, “Intracytoplasmic sperm injection in the mouse,” Biology of Reproduction, Vol.52, No.4, p. 709, 1995.

[3] [3] T. Arai, “Automated Embryonic Cell Manipulation Using Micro Robotics Technology,” IEEE Int. Conf. on Intelligent Robotics and Systems (IROS), in Workshop “Bio-medical Micro-Nano Robotics: New trends and future aspects in the next decade,” 2007.

[4] [4] A. Hasegaw, H. Uvet, K. Ohara, T. Takubo, Y. Mae, and T. Arai, “Self-controlled cell supply system for somatic cell cloning work in microfluidic chip,” MicroTAS2009, pp. 1255-1257, 2009.

[5] [5] A. Ichikawa, T. Tanikawa, K. Matsukawa, S. Takahashi, and K. Ohba, “Fluorescent Monitoring Using Microfludics Chip and Development of Syringe Pump for Automation of Enucleation to Automate Cloning,” IEEE Int. Conf. on Robotics and Automation (ICRA), pp. 2231-2236, 2009.

[6] [6] S. Sakuma, Y. Yamanishi, and F. Arai, “Magnetically Driven Microtools Actuated by a Focused Magnetic Field for Separating of Micro-particles,” J. of Robotics and Mechatronics, Vol.21, No.2, pp. 209-215, 2009.

[7] [7] Y. Yamanishi, S. Sakuma, K. Onda, and F. Arai, “Biocompatible Polymeric Magnetically Driven Microtool for Particle Sorting,” J. of Micro and Nano Mechatronics, Vol.4, No.1, pp. 49-57, 2008.

[8] [8] O. Satoh, A. Nakayama, S. Yamada, F. Arai, Y. Yamanishi, and S. Sakuma, “Investigation of On-chip Cell Coupling Module in Microfluidic Environments,” System Integration (SI2008), pp. 593-594, 2008.

[9] [9] H. Aso, M. Goto, S. Yamada, F. Arai, and S. Sakuma, “The cell fusion module using minute channel environment,” System Integration (SI2008), pp. 595-596, 2008.

[10] [10] U. Zimmerman and J. Vienken, “Electric field-induced cell-to-cell fusion,” J. Membrane Biol., Vol.67, pp. 165-182, 1982.

[11] [11] M. Yamada, K. Kano, Y. Tsuda, J. Kobayashi, M. Yamato, M. Seki, and T. Okano, “Microfluidic devices for size-dependent separation of liver cells,” Biomedical Microdevices, Vol.9, pp. 637-645, 2007.

[12] [12] M. Yamada, J. Kobayashi, M. Yamato, M. Seki, and T. Okano, “Millisecond treatment of cells using microfluidic devices via twostep carrier-medium exchange,” Lab on a Chip, Vol.8, pp. 772-778, 2008.

Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation

Yoko Yamanishi*1, Shinya Sakuma*2, Tomohiro Iyanagi*2, Fumihito Arai*3, Tatsuo Arai*4, Akiyuki Hasegawa*4, Tamio Tanikawa*5, Akihiko Ichikawa*5, Osamu Satoh*6, Akihiro Nakayama*6, Hiroshi Aso*7, Mitsuhiro Goto*7, Seiya Takahashi98, and Kazutsugu Matsukawa*8

Yoko Yamanishi^1, Shinya Sakuma^2, Tomohiro Iyanagi^2,
Fumihito Arai^3, Tatsuo Arai^4, Akiyuki Hasegawa^4,
Tamio Tanikawa^5, Akihiko Ichikawa^5, Osamu Satoh^6,
Akihiro Nakayama^6, Hiroshi Aso^7, Mitsuhiro Goto^7,
Seiya Takahashi⁹⁸, and Kazutsugu Matsukawa^*8