Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Manipulation by Optical Tweezers
Hisataka Maruyama*, Ryo Iitsuka**, Kazuhisa Onda**,
and Fumihito Arai*
*Department of Mechanical Science & Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
**Department of Bioengineering and Robotics, Tohoku University, 6-6-01 Aramaki-Aoba, Aoba-ku, Sendai 980-8579, Japan
Production of functional microtools having an arbitrary shape by self-assembly of microparticles and heat treatment above the glass transition temperature of the microparticles was developed. Polystyrene microbeads were used as a material of the microtool. A solution including microparticles was dispersed onto the silicon substrate having microtool patterns fabricated by photolithography and etching. Dispersed particles were introduced to the pattern by gravity force. Microparticles in the pattern aggregate autonomously by surface tension through evaporation of the solution. Aggregated microparticles were fused by heating above the glass transition temperature (100°C). Fused microparticles were detached from the pattern by ultrasonic treatment and used as microtools. Produced microtool has spherical part since the microtool is made of microparticles. Spherical part is suitable for trapping point of optical tweezers. We demonstrated production of microtools using self-assembly and manipulation of the fabricated microtool on a chip.
-  A. Fuchs et al., “A microelectronic chip opens new fields in rare cell population analysis and individual cell biology,” Proc. Micro Total Analysis system, California, USA, pp. 911-914, 2003.
-  F. Arai, T. Endo, R. Yamauchi, and T. Fukuda, “3D 6DOF Manipulation of Microbead by Laser Tweezers,” J. of Robotics and Mechatronics, Vol.18, No.2, pp. 153-159, 2006
-  A. Ashkin, “Acceleration and trapping of particles by radiation pressure,” Phys. Rev. Lett., Vol.24, No.4, pp. 156-159, 1970.
-  A. Ashkin and J. M. Dziedzic, “Internal cell manipulation using infrared laser traps,” Proc. Natl. Acad. Sci. USA., Vol.86, pp. 7914-7918, 1989.
-  A. Ashkin et al., “Observation of a single-beam gradient force optical trap for dielectric particles,” Opt. Lett., Vol.11, No.5, pp. 288-290, 1986.
-  D. G. Grier, “A revolution in optical manipulation,” Nature, Vol.424, pp. 21-27, 2003.
-  F. Arai et al., “3D Manipulation of Lipid Nanotubes with Functional Gel Microbeads,” Journal of Robotics and Mechatronics, Vol.19, No.2, pp. 198-204, 2007.
-  H. Maruyama, F. Arai, T. Fukuda, and T. Katsuragi, “Immobilization of individual cells by local photo polymerization on a chip,” The Analyst, Vol.130, No.3, pp. 304-310, 2005.
-  F. Arai et al., “Synchronized laser micromanipulation of multiple targets along each trajectory by single laser,” Appl. Phys. Lett., Vol.85, No.19, pp. 4301-4303, 2004.
-  H. Maruyama, T. Fukuda, and F. Arai, “Functional gel-microbead manipulated by optical tweezers for local environment measurement in mi-crochip,” Microfluidics and Nanofluidics, Vol.6, pp. 383-390, 2009.
-  D. Dendukuri et al., “Cntinuous-flow Lithography for Highthroughput Microparticle Synthesis,” Nature Materials, Vol.5, pp. 365-369, 2006
-  S. Maruo, O. Nakamura, and S. Kawata, “Three-dimensional Microfabrication with Two-photon-absorbed Photopolymerization,” Optics Lett., Vol.22, pp. 132-134, 1997.
-  F. Arai, K. Onda, R. Iitsuka, and H. Maruyama, “Multi-beam Laser Micromanipulation of Microtool, by Integrated Optical Tweezers,” Proc. of ICRA2009, pp. 1832-1837, 2009, .
-  R. L. Eriksen, P. C. Mogensen, and J. Glückstad, “Multiplebeam optical tweezers generated by the generalized phase-contrast method,” Opt. Lett., Vol.27, No.4, pp. 267-269, 2002
-  M. Capitanio, G. Romano, R. Ballerini, M. Giuntini, D. Dunlap, and L. Finzi, “Calibration of optical tweezers with differential interference contrast signals,” Review of Scientific Instruments, Vol.73, pp. 1687-1696, 2002
-  Y. Yin and Y. Xia: “Self-Assembly of Monodispersed Spherical Colloids into Complex Aggregates with Well-Defined Sizes, Shapes, and Structures,” Advanced Materials, Vol.13, No.4, pp. 267-271, 2001.
-  T. Onodera et al., “Ordered Array of Polymer Microspheres on Patterned Silicon Substrate Fabricated Using Step-by-Step Deposition Method,” Japanese J. of Applied Physics, Vol.47, No.2, pp. 1404-1407, 2008.
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