Multiscale 2D-SPR Biosensing for Cell Chips
Masayasu Suzuki*, Toyohiro Ohshima*, Shintaro Hane*,
Yasunori Iribe*, and Tatsuya Tobita**
*Dept. of Electric & Electronic Eng., University of Toyama, 3190 Gofuku, Toyama, Toyama 930-8555, Japan
**NTT Advanced Technology Corporation, 3-1 Morinosato, Wakamiya, Atsugi, Kanagawa 243-0124, Japan
Evaluating cell activity and functions in different-sized cell chambers requires multiscale sensing. We have been developing multiscale biosensing applied from 10 µm to 1 mm. We measured mouse IgG in micro wells using a high-resolution two-dimensional surface plasmon resonance (SPR) imaging affinity sensor. This sensor uses high refractive optics, a 1X to 7X microscopic lens, and a cooled CCD camera. The micro-well array was prepared with a PDMS film on gold sensor film. Protein A immobilized on sensor film was used for IgG recognition. SPR sensitivity was dramatically decreased with 10 and 8.5 µm microwells. To improve sensor sensitivity, we optimized the sensor’s measurement angle and exposure time, enabling mouse IgG to be detected in wells of 1 mm, 30 µm, and 10 µm using the same 2D-SPR imaging sensor and measurement protocol. These results show the feasibility of multiscale biosensing use in antibody production in a micro well or a cell chamber.
Yasunori Iribe, and Tatsuya Tobita, “Multiscale 2D-SPR Biosensing for Cell Chips,” J. Robot. Mechatron., Vol.19, No.5, pp. 519-523, 2007.
-  H. Andersson and A. van den Berg (Eds.), “Lab-on-Chips for Cellomics,” Kluwer, 2004.
-  M. Suzuki and Y. Iribe, “Multi-scale biosensing techniques for cell chips,” Proc. of MHS 2006, pp. 109-111, 2006.
-  L. C. Taylor and D. R. Walt, “Application of high-density optical microwell arrays in a live-cell biosensing system,” Anal. Biochem., Vol.278, pp. 132-142, 2000.
-  Y. Wakamoto, S. Umehara, K. Matsumura, I. Inoue, and K. Yasuda, “Development of non-destructive, non-contact single-cell based differential cell assay using on-chip microcultivation and optical tweezers,” Sensors and Actuators, Vol.B96, pp. 693-700, 2003.
-  M. Suzuki, “Development of a single cell screening system based on micro-arrayed cell chips,” J. Clin. Exp. Med. (IGAKU NO AYUMI), Vol.218, No.2, pp. 135-138, 2006 (in Japanese).
-  Toyama Medical-Bio Cluster
( http://www.tombic.net/ ).
-  S. Yamamura, H. Kishi, Y. Tokimitsu, S. Kondo, R, Honda, R. S. Ramachandra, M. Omori, E. Tamiya, and A. Muraguchi, “A single-cell microarray for analyzing cellular response,” Anal Chem., Vol.77, pp. 8050-8056, 2005.
-  M. Suzuki, H. Nakabayashi, Y. Jing, and M. Honda, “Optical pH and oxygen sensing for micro-arrayed cell chips,” Micro Total Analysis Systems 2005, Vol.2, pp. 1482-1484, 2005.
-  M. Suzuki, S. Hane, T. Ohshima, Y. Iribe, and T. Tobita, “Detection of antibodies in 10 μm wells on micro-arrayed cell chips by 2DSPR affinity imaging,” Micro Total Analysis Systems 2006, Vol.1, pp. 756-758, 2006.
-  J. M. Brockman, A. G. Frutos, and R. M. Corn, “A multistep chemical modification procedure to creat DNA arrays on gold surfaces for the study of protein-DNA interactions with surface plasmon resonance imaging,” J. Am. Chem. Soc., Vol.121, pp. 8044-8051, 1999.
-  E. Kretschmann and H. Raether, “Radiative decay of nonradiative surface plasmons excited by light,” Z. Naturforsch., Vol.23, pp. 2135-2136, 1968.
-  T. Tobita, H. Tabei, Y. Iwasaki, O. Niwa, K. Kurihara, and K. Suzuki, “Development of 2D-SPR measuring instrument and application to multi-channel measurement,” IEICE Technical Report, Vol.100, No.400, pp. 51-56, 2000 (in Japanese).
-  M. Hide, T. Tsutsui, H. Sato, T. Nishimura, K. Morimoto, S. Yamamoto, and K. Yoshizato, “Real-time analysis of ligand-induced cell surface and intracellular reactions of living mast cells using a surface plasmon resonance-based biosensor,” Anal. Biochem., Vol.302, pp. 28-37, 2002.
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