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JRM Vol.19 No.5 pp. 519-523
doi: 10.20965/jrm.2007.p0519
(2007)

Paper:

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Multiscale 2D-SPR Biosensing for Cell Chips

Masayasu Suzuki*, Toyohiro Ohshima*, Shintaro Hane*,
Yasunori Iribe*, and Tatsuya Tobita**

*Dept. of Electric & Electronic Eng., University of Toyama, 3190 Gofuku, Toyama, Toyama 930-8555, Japan

**NTT Advanced Technology Corporation, 3-1 Morinosato, Wakamiya, Atsugi, Kanagawa 243-0124, Japan

Received:
April 24, 2007
Accepted:
June 14, 2007
Published:
October 20, 2007
Creative Commons License
Keywords:
surface plasmon resonance, SPR imaging, cell chip, micro-array, IgG
Abstract
Evaluating cell activity and functions in different-sized cell chambers requires multiscale sensing. We have been developing multiscale biosensing applied from 10 µm to 1 mm. We measured mouse IgG in micro wells using a high-resolution two-dimensional surface plasmon resonance (SPR) imaging affinity sensor. This sensor uses high refractive optics, a 1X to 7X microscopic lens, and a cooled CCD camera. The micro-well array was prepared with a PDMS film on gold sensor film. Protein A immobilized on sensor film was used for IgG recognition. SPR sensitivity was dramatically decreased with 10 and 8.5 µm microwells. To improve sensor sensitivity, we optimized the sensor’s measurement angle and exposure time, enabling mouse IgG to be detected in wells of 1 mm, 30 µm, and 10 µm using the same 2D-SPR imaging sensor and measurement protocol. These results show the feasibility of multiscale biosensing use in antibody production in a micro well or a cell chamber.
Cite this article as:
M. Suzuki, T. Ohshima, S. Hane, Y. Iribe, and T. Tobita, “Multiscale 2D-SPR Biosensing for Cell Chips,” J. Robot. Mechatron., Vol.19 No.5, pp. 519-523, 2007.
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