Seed varieties are often differentiated via the manual and subjective classification of their external textural, spectral, and morphological biosignatures. This traditional method of manually inspecting seeds is inefficient and unreliable for seed phenotyping. The application of computer vision is an ideal solution allied with computational intelligence. This study used Lactuca sativa seed variants, which are commercially known as grand rapid, Chinese loose-leaf, and iceberg (which serves as noise data for extended model evaluation), in determining their corresponding classifications based on the extended morphological phenes using computational intelligence. Red-green-blue (RGB) imaging was employed for individual kernels. Extended morphological phenes, that is, solidity, roundness, compactness, and shape factors, were computed based on seed architectural traits and used as predictors to discriminate among the three cultivars. The suitability of ANFIS, NB, and CT was explored using a limited dataset. A mean accuracy of 100% was manifested in ANFIS; thus, it was proved to be the most reliable model.

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