IJAT Vol.12 No.1 pp. 37-44
doi: 10.20965/ijat.2018.p0037


On-Chip FRET Graphene Aptasensor

Yuko Ueno*,† and Kazuaki Furukawa**

*NTT Basic Research Laboratories, NTT Corporation
3-1 Morinosato, Wakamiya, Atsugi, Kanagawa 243-0198, Japan

Corresponding author

**Meisei University, Tokyo, Japan

May 23, 2017
November 23, 2017
January 5, 2018
graphene, aptamer, biosensor, microchannel, fluorescence resonance energy transfer

We review the recent advances in the use of our originally developed on-chip graphene aptasensor to detect biologically important proteins, such as cancer markers. The detection mechanism, based on fluorescence resonance energy transfer (FRET), occurs at a graphene–biomolecule interface. In our system, the graphene surface is modified with a pyrene–aptamer–dye probe. Pyrene functions as a linker to the graphene surface, the aptamer as a probe for selective protein recognition, and the dye as a fluorescence detection tag. Here, graphene behaves simultaneously as both an efficient acceptor for FRET over the entire visible region and as a strong adsorbate for single-stranded DNA (ssDNA), such as aptamers, via π-π interactions in the sp2 domain. The system allows us to perform molecular detection on a solid surface, which is advantageous for realizing on-chip sensors. Such on-chip sensors allow parallel analysis systems, such as array sensors. This enables the quantitative comparison of different samples by forming a multichannel configuration and/or a micropattern with different probes. Moreover, detecting the target protein is possible simply by adding a sample of less than 1 μL to the on-chip sensor; detection is completed in approximately 1 min. Aptasensors can be used for the detection of many different targets simply by replacing the aptamers. The simultaneous detection of multiple target molecules on a single chip using a 2 × 3 linear-array aptasensor was demonstrated here. Improved sensitivity was observed when a DNA spacer was incorporated into the aptamer, demonstrating that the probe can be modified in interesting ways.

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Cite this article as:
Yuko Ueno and Kazuaki Furukawa, “On-Chip FRET Graphene Aptasensor,” Int. J. Automation Technol., Vol.12, No.1, pp. 37-44, 2018
Yuko Ueno and Kazuaki Furukawa, Int. J. Automation Technol., Vol.12, No.1, pp. 37-44, 2018

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Last updated on Jun. 22, 2018