Review:
In Vitro Diagnostic Device with a Bio-Coupled Gate Field Effect Transistor
Toshiya Sakata†
Department of Materials Engineering, School of Engineering, The University of Tokyo
7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
†Corresponding author
In this study, we report a simple and rapid biosensing method for the analysis and in vitro monitoring of biological processes, including DNAbinding events, antigen-antibody interactions, and cellular functions, using a semiconductor device. Most biological phenomena involve cell-cell communication processes that are mediated by the transport of sodium or potassium ions and other charged biomolecules, such as DNA, across ion channels in the cell membrane. Therefore, our approach focused on the direct detection of changes in ion concentrations by utilizing a semiconductor-based biosensor device. Our results demonstrated that our semiconductor-based biosensor platform achieves label-free and noninvasive biosensing that is suitable for in vitro diagnosis.
- [1] G. E. Moore, “Cramming More Components Onto Integrated Circuits,” Proc. of the IEEE, Vol.86, pp. 82-85, 1998.
- [2] Y. Rondelez, G. Tresset, T. Nakashima, Y. Kato-Yamada, H. Fujita, S. Takeuchi, and H. Noji, Nature, “Highly coupled ATP synthesis by F1-ATPase single molecules,” Vol.433, pp. 773-777, 2005.
- [3] P. Bergveld, “Development of an Ion-Sensitive Solid-State Device for Neurophysiological Measurements,” IEEE Trans. Biomed. Eng., Vol.17, pp. 70-71, 1970.
- [4] P. T. McBride, J. Janata, P. A. Comte, S. D. Moss, and C. C. Johnson, “Ion-selective field effect transistors with polymeric membranes,” Anal. Chim. Acta, Vol.101, pp. 239-245, 1978.
- [5] T. Sakata and Y. Miyahara, “DNA sequencing based on intrinsic molecular charges,” Angewandte Chemie Int. Edition, Vol.45, pp. 2225-2228, 2006.
- [6] T. Sakata and Y. Miyahara, “Direct transduction of primer extension into electrical signal using genetic field effect transistor,” Biosensors and Bioelectronics, Vol.22, pp. 1311-1316, 2007.
- [7] T. Sakata, M. Ihara, I. Makino, Y. Miyahara, and H. Ueda, “Open sandwich-based immuno-transistor for label-free and highly sensitive detection of low molecular weight antigen,” Analytical Chemistry, Vol.81, pp. 7532-7537, 2009.
- [8] T. Sakata, A. Saito, J. Mizuno, H. Sugimoto, K. Noguchi, E. Kikuchi, and H. Inui, “Single embryo-coupled gate field effect transistor for elective single embryo transfer,” Analytical Chemistry, Vol.85, pp. 6633-6638, 2013.
- [9] T. Sakata, K. Nishimura, Y. Miyazawa, A. Saito, H. Abe, and T. Kajisa, “Ion sensitive transparent-gate transistor for visible cell sensing,” Analytical Chemistry, Vol.89, pp. 3901-3908, 2017.
- [10] T. Kajisa and T. Sakata, “Glucose responsive hydrogel electrode for biocompatible glucose transistor,” Sci. Technol. Adv. Mater., Vol.18, pp. 26-33, 2017.
- [11] Y. Maekawa, Y. Shibuta, and T. Sakata, “Charge Behaviors around Oxide Device/Pseudo-Physiological Solution Interface with Molecular Dynamic Simulations,” Japanese Journal of Applied Physics, Vol.52, 127001, 2013
- [12] Y. Maekawa, Y. Shibuta, and T. Sakata, “Distinctive potential behavior at oxidized surface of semiconductor device in concentrated aqueous salt solution,” ChemElectroChem, Vol.1, pp. 1516-1524, 2014.
- [13] Y. Maekawa, Y. Shibuta, and T. Sakata, “Effect of double-stranded DNA on electrical double layer structure atoxide/electrolyte interface in classical molecular dynamics simulation,” Chem. Phys. Lett., Vol.619, pp. 152-157, 2015.
- [14] B. M. Lowe, Y. Maekawa, Y. Shibuta and T. Sakata, “Dynamic behaviour of the silica-water-bio electrical double layer in the presence of a divalent electrolyte,” C-K. Skylaris, and N. G. Green, Physical Chemistry Chemical Physics, Vol.19, pp. 2687-2701, 2017.
- [15] K. Sakamaki, T. M. Ishii, T. Sakata, K. Takemoto, C. Takagi, A. Takeuchi, R. Morishita, K. Chiba, H. Sugimoto, S. Tamate, Y. Satou, S. Matsuoka, K. Koyamada, T. Sawasaki, T. Nagai, and N. Ueno, “Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage,” BBA - Molecular Cell Research, Vol.1863, pp. 2766-2783, 2016.
- [16] T. Sakata and Y. Matsuse, “In situ electrical monitoring of cancer cells invading vascular endothelial cells with semiconductor-based biosensor,” Genes to Cells, Vol.22, pp. 203-209, 2017.
This article is published under a Creative Commons Attribution-NoDerivatives 4.0 Internationa License.