IJAT Vol.9 No.5 pp. 534-540
doi: 10.20965/ijat.2015.p0534


Study on Nanoparticle Sizing Using Fluorescent Polarization Method with DNA Fluorescent Probe

Terutake Hayashi*, Yuki Ishizaki**, Masaki Michihata**, Yasuhiro Takaya**, and Shin-ichi Tanaka***

*Kyushu University
744 Motooka, Nishiku, Fukuoka, Fukuoka 819-0395, Japan

**Osaka University
2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

***Kure National College of Technology
2-2-11 Agaminami, Kure, Hiroshima 737-8506, Japan

March 24, 2015
May 7, 2015
September 5, 2015
nanoparticle sizing, fluorescent DNA probe, gold nanoparticle, fluorescent polarization, rotational diffusion coefficient

Fluorescent polarization methods are used to detect complementary base pairing of DNA in biological fields. These methods work by measuring the rotational diffusion coefficient of Brownian motion of the fluorescent particles in solution. The rotational diffusion coefficient corresponds to the inverse third power of diameter according to the Debye-Stokes-Einstein equation for nanoparticles as hard spheres. We develop a novel method to measure the rotational diffusion coefficient using a fluorescent probe with a DNA spacer connected to a gold nanoparticle. We studied the physical characteristics of this probe to verify the feasibility of the proposed method. The rotational diffusion coefficients of gold nanoparticles with diameters ranging between 5–20 nm were measured using this developed system. In this manuscript we describe a novel fluorescent polarization method for nanoparticle sizing using a fluorescent DNA probe.

Cite this article as:
Terutake Hayashi, Yuki Ishizaki, Masaki Michihata, Yasuhiro Takaya, and Shin-ichi Tanaka, “Study on Nanoparticle Sizing Using Fluorescent Polarization Method with DNA Fluorescent Probe,” Int. J. Automation Technol., Vol.9, No.5, pp. 534-540, 2015.
Data files:
  1. [1]  R. G. Freeman et al., “Self-assembled metal colloid monolayers: An approach to SERS substrates,” Science, Vol.267, No.5204, pp. 1629-1632, 1995.
  2. [2]  S. Sun et al., “Monodisperse FePt nanoparticles and ferromagnetic FePt nanocrystal superlattices,” Science, Vol.287, No.5460, pp. 1989-1992, 2000.
  3. [3]  D. L. Feldheim et al., “Electron transfer in self-assembled inorganic polyelectrolyte/metal nanoparticle heterostructures,” J. Am. Chem. Soc., Vol.118, No.32, pp. 7640-7641, 1996.
  4. [4]  R. P. Andres et al., “Self-assembly of a two-dimensional superlattice of molecularly linked metal clusters,” Science, Vol.273, pp. 1690-1693, 1987.
  5. [5]  A. N. Shipway et al., “Nanoparticle arrays on surfaces for electronic, optical, and sensor applications,” Chem. Phys. Chem., Vol.1, pp. 18-52, 2000.
  6. [6]  P. Debye, “Polar Molecules,” Dover, 1929.
  7. [7]  P. P. Jose et al., “Complete breakdown of the Debye model of rotational relaxation near the isotropic-nematic phase boundary: Effects of intermolecular correlations in orientational dynamics,” Phys. Rev. E, Vol.73, No.3, p. 031705, 2006.
  8. [8]  L. Andreozzi et al., “Evidence of a fractional Debye-Stokes-Einstein law in supercooled o-terphenyl,” Europhys. Lett., Vol.38, No.9, pp. 669-674, 1997.
  9. [9]  K. Kinosita et al., “A theory of fluorescence polarization decay in membranes,” Biophys. J., Vol.20, No.3, pp. 289-305, 1977.
  10. [10]  A. H. A. Clayton et al., “Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM),” Biophys. J., Vol.83, No.3, pp. 1631-1649, 2002.
  11. [11]  R. D. Spencer and G. Weber, “Measurements of subnanosecond fluorescence lifetimes with a cross-correlation phase fluorometer,” Annals of the New York Academy of Science, Vol.158, No.1, pp. 361-376, 1969.
  12. [12]  C. Vericat, et al., “Self-assembled monolayers of thiols and dithiols on gold: new challenges for a well-known system,” Chem. Soc. Rev., Vol.39, No.5, pp. 1805-1834, 2010.
  13. [13]  C. S. Yun et al., “Nanometal surface energy transfer in optical rulers breaking the FRET barrier,” J. Am. Chem. Soc., Vol.127, No.9, pp. 3115-3119, 2005.
  14. [14]  H. Morimura et al., “Nano-analysis of DNA conformation changes induced by transcription factor complex binding using plasmonic nanodimers,” ACS Nano., Vol.12, pp. 10733-10740, 2013.
  15. [15]  T. Sen et al., “Surface energy transfer from rhodamine 6G to gold nanoparticles A spectroscopic ruler,” Applied physics letters, Vol.91, p. 1033, 2007.
  16. [16]  S. J. Hurst et. al., “Maximizing DNA loading on a range of gold nanoparticle sizes.,” Anal. Chem., Vol.78, No.24, pp. 8313-8318, 2006.
  17. [17]  M. N. Bui et al., “Gold nanoparticle aggregation-based highly sensitive DNA detection using atomic force microscopy,” Anal. Bioanal. Chem, Vol.388, pp. 1185-1190, 2007.

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