JRM Vol.35 No.5 pp. 1158-1164
doi: 10.20965/jrm.2023.p1158


Deformation and Trapping of Cell Nucleus Using Micropillar Substrates Possibly Affect UV Radiation Resistance of DNA

Kazuaki Nagayama, Chiaki Sagawa, and Akiko Sato

Micro-Nano Biomechanics Laboratory, Department of Mechanical Systems Engineering, Ibaraki University
4-12-1 Nakanarusawa-cho, Hitachi, Ibaraki 316-8511, Japan

March 24, 2023
August 2, 2023
October 20, 2023
cell biomechanics, mechanobiology, mechanotransduction, DNA damages, microfabrication

DNA damage induced by the ultraviolet (UV) light, which affects adversely on genome stability, causes many kinds of diseases. Thus, a biochemical or biomechanical method in DNA damage protection is well required. In the present study, we investigated the effects of mechanical factors, such as deformation of cell nucleus using polydimethylsiloxane (PDMS)-based microfabricated array of micropillars, on UV radiation resistance of DNA in cultured cells. The epithelial-like cells spread normally in the spaces between micropillars and their nuclei showed remarkable deformation and appeared to be “trapped” mechanically on the array of pillars. We found that the UV radiation-induced DNA damage estimated by the fluorescent intensity of the phospho-histone γ-H2AX, was significantly inhibited with the nucleus deformation on the pillars. The result indicates that the inhibition of UV radiation-induced DNA damages might be resulted from structural change of DNA caused by the mechanical stress of the cell nucleus on the micropillars.

Cell nucleus deformation on micropillars

Cell nucleus deformation on micropillars

Cite this article as:
K. Nagayama, C. Sagawa, and A. Sato, “Deformation and Trapping of Cell Nucleus Using Micropillar Substrates Possibly Affect UV Radiation Resistance of DNA,” J. Robot. Mechatron., Vol.35 No.5, pp. 1158-1164, 2023.
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