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JRM Vol.35 No.4 pp. 931-937
doi: 10.20965/jrm.2023.p0931
(2023)

Paper:

Quantitative Analysis of the Coordinated Movement of Cells in a Freely Moving Cell Population

Daiki Umetsu* ORCID Icon, Satoshi Yamaji**,***, Daiki Wakita** ORCID Icon, and Takeshi Kano** ORCID Icon

*Graduate School of Science, Osaka University
1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan

**Research Institute of Electrical Communication, Tohoku University
2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan

***Graduate School of Engineering, Tohoku University
6-6-01 Aramaki Aza-Aoba, Aoba-ku, Sendai, Miyagi 980-8579, Japan

Received:
March 2, 2023
Accepted:
April 26, 2023
Published:
August 20, 2023
Keywords:
self-propelled agent, velocity correlation, vector correlation, hemocyte, muscle remodeling
Abstract

Coordinated movement of self-propelled agents has been well studied in collectives or swarms that display directional movement. Self-propelled agents also develop stable spatial patterns in which the agents do not necessarily exhibit directional collective movement. However, quantitative measures that are required to analyze the local and temporal coordinated movements during pattern formation processes have not been well established. Here, we study the coordinated movement of individual pairs of two different types of cells in a freely moving cell population. We introduced three criteria to evaluate coordinated movement in live imaging data obtained from the abdomen of the fruit fly, Drosophila melanogaster, at the pupal stage. All three criteria were able to reasonably identify coordinated movement. Our analysis indicates that the combined usage of these criteria can improve the evaluation of whether a pair of cells exhibits coordinated movement or not by excluding false positives. Quantitative approaches to identifying coordinated movement in a population of freely moving agents constitute a key foundational methodology to study pattern formations by self-propelled agents.

Coordinated movement of migrating cells

Coordinated movement of migrating cells

Cite this article as:
D. Umetsu, S. Yamaji, D. Wakita, and T. Kano, “Quantitative Analysis of the Coordinated Movement of Cells in a Freely Moving Cell Population,” J. Robot. Mechatron., Vol.35 No.4, pp. 931-937, 2023.
Data files:
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Last updated on Feb. 19, 2024