Three-Dimensional Assembly of Multilayered Tissues Using Water Transfer Printing
Taisuke Masuda*, Yuka Yamagishi*, Natsuki Takei*,
Hirofumi Owaki*, Michiya Matsusaki**, Mitsuru Akashi**,
and Fumihito Arai*
*Department of Micro-Nano Systems Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
**Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
A rapid construction process is necessary to build up numerous cell modules into three-dimensional (3D) tissues that retain the tissue geometries and initial conditions of the cells. We propose a new 3D assembly technique using water transfer printing to fabricate a hollow tubular tissue structure. Utilizing this assembly technique, we discuss the relationship between the 3D transcriptional body of a gel matrix and the developed shape of transferred tissue. We then fabricate hollow tubular tissue. Simulation of the 3D environment in which tissues normally develop and function is crucial for the engineering of in vitro models that can be used for the formation of complex tissues. These artificial hollow tubular tissues could be used as in vitro simulators for drug efficiency evaluation and operative training.
Hirofumi Owaki, Michiya Matsusaki, Mitsuru Akashi, and
and Fumihito Arai, “Three-Dimensional Assembly of Multilayered Tissues Using Water Transfer Printing,” J. Robot. Mechatron., Vol.25, No.4, pp. 690-697, 2013.
-  H. Aubin, J.W. Nichol, C. B. Hutson et al., “Directed 3D cell alignment and elongation in microengineered hydrogels,” Biomaterials, Vol.31, No.27, pp. 6941-6951, 2010.
-  M. Papadaki, N. Bursac, R. Langer et al., “Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies,” American J. of Physiology-Heart and Circulatory Physiology, Vol.280, No.1, pp. H168-H178, 2001.
-  T. Anada, T. Kumagai, Y. Honda et al., “Dose-dependent osteogenic effect of octacalcium phosphate on mouse bone marrow stromal cells,” Tissue Engineering Part A, Vol.14, No.6, pp. 965-978, 2008.
-  A. Khademhosseini, J. P. Vacanti, and R. Langer, “Progress in Tissue Engineering,” Scientific American, Vol.300, No.5, pp. 64-71, 2009.
-  T. Masuda, T. Kawai, T. Anada et al., “Quality of regenerated bone enhanced by implantation of octacalcium phosphate-collagen composite,” Tissue Eng. Part C: Methods, Vol.16, No.3, pp. 471-478, 2010.
-  V. Mironov, R. P. Visconti, V. Kasyanov et al., “Organ printing: tissue spheroids as building blocks,” Biomaterials, Vol.30, No.12, pp. 2164-74, 2009.
-  A. P. Napolitano, D. M. Dean, A. J.Man et al., “Scaffold-free threedimensional cell culture utilizing micromolded nonadhesive hydrogels,” Biotechniques, Vol.43, No.4, pp. 494-500, 2007.
-  A. Khademhosseini, R. Langer, J. Borenstein et al., “Microscale technologies for tissue engineering and biology,” Proc. of the National Academy of Sciences of the United States of America, Vol.103, No.8, pp. 2480-2487, 2006.
-  T. Masuda, N. Takei, T. Nakano et al., “A microfabricated platform to form three-dimensional toroidal multicellular aggregate,” Biomedical Microdevices, Vol.14, No.6, pp. 1085-1093, 2012.
-  C. S. Chen, M. Mrksich, S. Huang et al., “Geometric control of cell life and death,” Science, Vol.276, No.5317, pp. 1425-1428, 1997.
-  R. McBeath, D. M. Pirone, C. M. Nelson et al., “Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment,” Developmental Cell, Vol.6, No.4, pp. 483-495, 2004.
-  J. A. Burdick and G. Vunjak-Novakovic, “Engineered Microenvironments for Controlled Stem Cell Differentiation,” Tissue Engineering Part A, Vol.15, No.2, pp. 205-219, 2009.
-  J. Fukuda, A. Khademhosseini, Y. Yeo et al., “Micromolding of photocrosslinkable chitosan hydrogel for spheroid microarray and co-cultures,” Biomaterials, Vol.27, No.30, pp. 5259-5267, 2006.
-  T.Masuda, I. Takahashi, T. Anada et al., “Development of a cell culture system loading cyclic mechanical strain to chondrogenic cells,” J. of Biotechnology, Vol.133, No.2, pp. 231-238, 2008.
-  C. M. Nelson, M. M. Vanduijn, J. L. Inman et al., “Tissue geometry determines sites of mammary branching morphogenesis in organotypic cultures,” Science, Vol.314, No.5797, pp. 298-300, 2006.
-  B. G. Chung, K. H. Lee, A. Khademhosseini et al., “Microfluidic fabrication of microengineered hydrogels and their application in tissue engineering,” Lab on a Chip, Vol.12, No.1, pp. 45-59, 2012.
-  J. W. Nichol, S. T. Koshy, H. Bae et al., “Cell-laden microengineered gelatin methacrylate hydrogels,” Biomaterials, Vol.31, No.21, pp. 5536-5544, 2010.
-  T. Anada, T. Masuda, Y. Honda et al., “Three-dimensional cell culture device utilizing thin membrane deformation by decompression,” Sensors and Actuators B: Chemical, Vol.147, No.1, pp. 376-379, 2010.
-  M. Matsusaki, H. Ajiro, T. Kida et al., “Layer-by-Layer Assembly Through Weak Interactions and Their Biomedical Applications,” Advanced Materials, Vol.24, No.4, pp. 454-474, 2012.
-  A. Nishiguchi, H. Yoshida, M. Matsusaki et al., “Rapid Construction of Three-Dimensional Multilayered Tissues with Endothelial Tube Networks by the Cell-Accumulation Technique,” Advanced Materials, Vol.23, No.31, pp. 3506-3510, 2011.
-  C. E. Ventura, “Experimental and applied mechanics, Vol.4,” Proc. of the 2012 annual conf. on experimental and applied mechanics, New York, NY: Springer Science+Business Media, LLC, 2012.
-  R. S. Ashton, A. Banerjee, S. Punyani et al., “Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture,” Biomaterials, Vol.28, No.36, pp. 5518-5525, 2007.
-  T. Takei, S. Sakai, T. Yokonuma et al., “Fabrication of artificial endothelialized tubes with predetermined three-dimensional configuration from flexible cell-enclosing alginate fibers,” Biotechnology Progress, Vol.23, No.1, pp. 182-186, 2007.
-  Y. Isobe, T. Kosaka, G. Kuwahara et al., “Oriented Collagen Scaffolds for Tissue Engineering,” Materials, Vol.5, No.3, pp. 501-511, 2012.
-  H. Mikami, G. Kuwahara, N. Nakamura et al., “Two-Layer Tissue Engineered Urethra Using Oral Epithelial and Muscle Derived Cells,” J. of Urology, Vol.187, No.5, pp. 1882-1889, 2012.
-  E. R. Ochoa and J. P. Vacanti, “An overview of the pathology and approaches to tissue engineering,” Lymphatic Continuum: Lymphatic Biology and Disease, Vol.979, pp. 10-26, 2002.
-  H. Owaki, T. Masuda, T. Kawahara et al., “All-in-one microfluidic device for microvascular connection,” Proc. of IEEE MEMS, pp. 1081-1084, 2013.
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