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JRM Vol.25 No.4 pp. 631-636
doi: 10.20965/jrm.2013.p0631
(2013)

Paper:

Preparation of Poly(N-isopropylacrylamide) Grafted Polydimethylsiloxane by Using Electron Beam Irradiation

Yoshikatsu Akiyama, Masayuki Yamato, and Teruo Okano

Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWMU), 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan

Received:
March 2, 2013
Accepted:
May 21, 2013
Published:
August 20, 2013
Keywords:
poly(N-isopropylacrylamide), poly(dimethylsiloxane), electron beam irradiation, temperature-responsive cell culture surface
Abstract

A poly(N-isopropylacrylamide) (PIPAAm) grafted poly(dimethylsiloxane) (PDMS) surface was prepared as a temperature-responsive cell culture surface by using electron beam (EB) irradiation. Different chemical treatments to modify the bare PDMS surface were investigated for subsequent grafting of PIPAAm, and treatment conditions were optimized to prepare the temperature-responsive cell culture surface. The PDMS surface was initially activated to form silanol groups with conventional O2 plasma or hydrochloric acid (HCl) treatment. Activated PDMS surfaces were individually immobilized with three different conventional silane compounds, i.e., 3-mercaptopropyltrimethoxysilane (MerTMS), 3-methacryloxypropyltrimethoxysilane (MetTMS), and 3-aminopropyltrimethoxysilane (AmiTMS). O2 plasma treatment made PDMS more hydrophilic. In contrast, PDMS surfaces activated with HCl treatment were relatively hydrophobic. Observation of the activated PDMS surface modified with MerTMS, MetTMS, and AmiTMS indicated that these silane compounds had been favorably immobilized on plasma-treated PDMS surfaces. FT-IR/ATR analysis demonstrated that immobilized silane compounds enabled PIPAAm grafting on the PDMS surface. Cell attachment and detachment analysis also suggested that the PDMS surface sequentially treated with O2 plasma and AmiTMS compound was a substrate appropriate for preparing a temperature-responsive cell culture surface by EB irradiation-induced PIPAAm grafting method. The intelligent surface may further be applied to mechanically stretchable temperature-responsive cell culture surfaces.

Cite this article as:
Yoshikatsu Akiyama, Masayuki Yamato, and Teruo Okano, “Preparation of Poly(N-isopropylacrylamide) Grafted Polydimethylsiloxane by Using Electron Beam Irradiation,” J. Robot. Mechatron., Vol.25, No.4, pp. 631-636, 2013.
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