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JRM Vol.25 No.2 pp. 277-284
doi: 10.20965/jrm.2013.p0277
(2013)

Paper:

Cellular Force Measurement Using a Nanometric-Probe-Integrated Microfluidic Chip with a Displacement Reduction Mechanism

Shinya Sakuma and Fumihito Arai

Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan

Received:
November 8, 2012
Accepted:
November 20, 2012
Published:
April 20, 2013
Keywords:
microrobot, magnetic actuator, microfluidic chip, cellular force measurement, oocyte
Abstract

This paper presents noncontact nanometric positioning of a probe tip with high output force in a microfluidic chip. To measure cellular force in a microfluidic chip on the basis of cell deformation, we employed an on-chip probe with a magnetic drive method with actuation on the order of millinewtons. A reduction mechanism was proposed to realize nanometric resolution for positioning the probe tip. This mechanism utilizes a combination of springs with different stiffness levels and is driven bymagnetic force. The performance of the prototype device was examined and results indicated that, as ameasure of repetitive positioning accuracy, standard deviation of probe tip displacement was under 0.18 µm. Deformation was successfully measured for an oocyte on the order of 0.1 mN, demonstrating, as a consequence, nanometric order noncontact actuation of the on-chip probe with high output force. Using this on-chip probe, cellular force measurement was achieved for the microfluidic chip.

Cite this article as:
Shinya Sakuma and Fumihito Arai, “Cellular Force Measurement Using a Nanometric-Probe-Integrated Microfluidic Chip with a Displacement Reduction Mechanism,” J. Robot. Mechatron., Vol.25, No.2, pp. 277-284, 2013.
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