single-rb.php

JRM Vol.22 No.5 pp. 639-643
doi: 10.20965/jrm.2010.p0639
(2010)

Paper:

Detection and Collection System of Target Single Cell Based on pH and Oxygen Sensing

Masayasu Suzuki, Hiroyuki Tanaka, and Yasunori Iribe

Department of Electric and Electronic Engineering, University of Toyama, 3190 Gofuku, Toyama, Toyama 930-8555, Japan

Received:
February 22, 2010
Accepted:
June 8, 2010
Published:
October 20, 2010
Keywords:
single cell, microarray, pH, oxygen, cell manipulation
Abstract

This paper describes single-cell-based detection and collection using pH and oxygen sensing with microarrayed chemical sensors we developed previously to monitor single-cell activity in parallel. Such sensors consist of optical sensor film for pH or oxygen and microwell arrays prepared with carbon-black-doped polydimethylsiloxane (PDMS). We monitored singlecell respiration in parallel using a microarrayed oxygen sensor. An automatic single-cell collector we developed can be used with a commercial inverted microscope. The single-cell-based detection and collection we developed based on respiration or metabolic activity combines these two techniques. Model experiments for single-cell-based detection and collection based on metabolic activity used urease-immobilized microbeads (6 µm i.d.). Fluorescence intensity after substrate injection increased only in wells containing urease-immobilized microbeads. Bead in target wells could be successfully collected alone, leaving other beads in their wells, by simply pushing a controller button, requiring no training or skill.

Cite this article as:
Masayasu Suzuki, Hiroyuki Tanaka, and Yasunori Iribe, “Detection and Collection System of Target Single Cell Based on pH and Oxygen Sensing,” J. Robot. Mechatron., Vol.22, No.5, pp. 639-643, 2010.
Data files:
References
  1. [1] H. Andersson and A. Berg (Eds.), “Lab-on-Chips for Cellomics,” Kluwer, 2004.
  2. [2] M. Suzuki and Y. Iribe, “Multi-scale Biosensing Techniques for Cell Chips,” Proc. of MHS 2006, pp. 392-395, 2006.
  3. [3] M. Suzuki, H. Nakabayashi, Y. Jing, and M. Honda, “Optical pH and oxygen sensing for micro-arrayed cell chips,” Micro Total Analysis Systems 2005, Vol.2, pp. 1482-1484, 2005.
  4. [4] M. Suzuki, T. Ohshima, and Y. Iribe, “2D-SPR Affinity Biosensing in 10 µm Wells for Multi-scale biosensing,” Proc. of MHS 2007, pp. 133-137, 2007.
  5. [5] M. Suzuki, Y. Iribe, and T. Tobita, “Surface Plasmon Resonance Array Devices,” Handbook of Biosensors and Biochips (R.S.Marks et al. Eds.), Wiley, Vol.2, pp. 917-924, 2007.
  6. [6] S. Yamamura, H. Kishi, Y. Tokimitsu, S. Kondo, R. Honda, R. S. Ramachandra, M. Omori, E. Tamiya, and A. Muraguchi, “Single-cell microarray for analyzing cellular response,” Anal. Chem., Vol.77, pp. 8050-8056, 2005.
  7. [7] K. Tajiri, H. Kishi, Y. Tokimitsu, S. Kondo, T. Ozawa, K. Kinoshita, A. Jin, S. Kadowaki, T. Sugiyama, and A. Muraguchi, “Cell microarray analysis of antigen-specific B-cell,” Cytometry A, Vol.71A, pp. 961-967, 2007.
  8. [8] J. R. Epstein and D. R. Walt, “Fluorescence-based fibre optic arrays: a universal platform for sensing,” Chem. Soc. Rev., Vol.32, pp. 203-214, 2003.
  9. [9] L. C. Taylor and D. R. Walt, “Application of high-density optical microwell arrays in a live-cell biosensing systems,” Anal. Biochem., Vol.278, pp. 132-142, 2000.
  10. [10] I. Biran and D. R. Walt, “Optical imaging fiber-based single live cell arrays: a high density cell assay platform,” Anal. Chem., Vol.74, pp. 3046-3054, 2002.
  11. [11] Y. Kuang, I. Biran, and D. R. Walt, “Living bacteria cell arrays for genotoxin monitoring,” Anal. Chem., Vol.76, pp. 2902-2909, 2004.
  12. [12] D. D. Bernhard, S. Mall, and P. Pantano, “Fabrication and characterization of microwell array chemical sensors,” Anal. Chem., Vol.73, pp. 2484-2490, 2001.
  13. [13] M. Suzuki, T. Yamada, S. Kato, and Y. Iribe, “Detection of Single Cell Activity by Using Fluorescence-Based Multiscale pH and Oxygen Sensors,” Proc. of MHS 2008, pp. 311-316, 2008.
  14. [14] M. C. Moreno-Bondi, O. S. Wolfbeis, M. J. P. Leiner, and B. P. H. Schaffar, “Oxygen optrode for use in a fiber-optic glucose biosensor,” Anal. Chem., Vol.62, pp. 2377-2380, 1990.

*This site is desgined based on HTML5 and CSS3 for modern browsers, e.g. Chrome, Firefox, Safari, Edge, Opera.

Last updated on Oct. 28, 2021