Detection and Collection System of Target Single Cell Based on pH and Oxygen Sensing
Masayasu Suzuki, Hiroyuki Tanaka, and Yasunori Iribe
Department of Electric and Electronic Engineering, University of Toyama, 3190 Gofuku, Toyama, Toyama 930-8555, Japan
This paper describes single-cell-based detection and collection using pH and oxygen sensing with microarrayed chemical sensors we developed previously to monitor single-cell activity in parallel. Such sensors consist of optical sensor film for pH or oxygen and microwell arrays prepared with carbon-black-doped polydimethylsiloxane (PDMS). We monitored singlecell respiration in parallel using a microarrayed oxygen sensor. An automatic single-cell collector we developed can be used with a commercial inverted microscope. The single-cell-based detection and collection we developed based on respiration or metabolic activity combines these two techniques. Model experiments for single-cell-based detection and collection based on metabolic activity used urease-immobilized microbeads (6 µm i.d.). Fluorescence intensity after substrate injection increased only in wells containing urease-immobilized microbeads. Bead in target wells could be successfully collected alone, leaving other beads in their wells, by simply pushing a controller button, requiring no training or skill.
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