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JRM Vol.22 No.2 pp. 197-203
doi: 10.20965/jrm.2010.p0197
(2010)

Paper:

Optimizing the Conditions for pH Measurement with an Automated pH Measurement System Using a Flow-Through-Type Differential Sensor Probe Consisting of pH-FETs

Akira Yamada*,**, Satoshi Mohri***, Michihiro Nakamura*,
and Keiji Naruse*

*Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama-city, Okayama 700-8558, Japan

**Department of Mechanical Systems Engineering, Faculty of Engineering, Hiroshima Institute of Technology (Current address), 2-1-1 Miyake, Saeki-ku, Hiroshima 731-5193, Japan

***Physiology 1, Kawasaki Medical School, 577 Matsushima, Kurashiki-city, Okayama 701-0192, Japan

Received:
October 1, 2009
Accepted:
January 25, 2010
Published:
April 20, 2010
Keywords:
ISFET, automation, optimization, differential pH sensor probe, 96-well microplate
Abstract

In previous papers we have reported on the construction of a flow-through-type differential pH sensor probe for a fully automated pH measurement system. Our flow-through-type sensor probe is quite different from the dipping-type sensor probes now in common use. To realize highly accurate and high-speed measurements with our probe, it is essential to optimize measurement conditions such as the volume of the sample solution suctioned into the probe, the washing time and related parameters, and the measurement time required for stabilization of the electrode potential. In this paper we describe a method to derive suitable measurement conditions for a pH measurement system equipped with a flow-through-type differential sensor probe consisting of pH-sensitive Field-Effect Transistors (pH-FETs). After establishing the optimum measurement conditions, we confirmed the performance of our system for the pH measurement of 26 μl solutions over a 106 sec cycle. The measurement error with diluted low-buffering-capacity solutions was on the order of 0.01 pH.

Cite this article as:
Akira Yamada, Satoshi Mohri, Michihiro Nakamura, and
and Keiji Naruse, “Optimizing the Conditions for pH Measurement with an Automated pH Measurement System Using a Flow-Through-Type Differential Sensor Probe Consisting of pH-FETs,” J. Robot. Mechatron., Vol.22, No.2, pp. 197-203, 2010.
Data files:
References
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Last updated on Mar. 05, 2021