Fabrication of Cell-Adhesion Surface and Arteriole Model by Photolithography
Fumihito Arai*, Takuma Nakano*, Mika Tada*, Yu-Ching Lin*,
Seiichi Ikeda**, Tomoyuki Uchida**, Hiroyuki Oura**,
Toshio Fukuda**, Takehisa Matsuda***, and Makoto Negoro****
*Department of Bioengineering and Robotics, Tohoku University, 6-6-01 Aramaki-Aoba, Aoba-ku, Sendai 980-8579, Japan
**Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
***Kanazawa Institute of Technology, 3-1 Yasukaho, Hakusan, Ishikawa 924-0838, Japan
****Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan
We have been developing scaffolds of three-dimensional (3D) synthetic vascular prosthesis in tailor-made. Human umbilical vein endothelial cells (HUVECs) attached on the inner surface of the scaffold have anticoagulant effects. Asperity structures of the inner surface are important to cell adhesion. It is important to quantify the inner surface asperity condition of the scaffold by observing HUVECs behavior and morphology. For this purpose, we recreated the inner surface profile of the scaffold on a poly(dimethilsiloxane) (PDMS) substrate by microfabrication. We made semiround convex patterns of resist that had 8 µm in diameter and 5 µm high using photolithography, and the concave pattern on the PDMS substrate by printing. We observed HUVECs adhering to the PDMS substrate having concave pattern on it surface. The distribution density of the concaves of the tested pattern is 1600 /mm2 or 40,000 in a 25 mm2 area. In addition, we fabricated an arteriole model by photolithography, creating an arteriole tube model that had 1.1 cm long and 300-400 µm in diameter. We confirmed that the arteriole model had no leakage using a methylene blue solution flow in the channel.
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