JRM Vol.18 No.6 pp. 827-830
doi: 10.20965/jrm.2006.p0827


Novel Computed Tomography for Reconstructing Three-Dimensional Phase Contrast Images of Single Living Cells

Yusuke Inoue*, Ichirou Ishimaru**, Toshiki Yasokawa**,
Katsumi Ishizaki**, and Makoto Yoshida**

*Fuji Xerox Corporation, 2-17-22 Akasaka, Minato-ku, Tokyo 107-0052, Japan

**Faculty of Engineering, Kagawa University, 2217-20 Hayashi-cho, Takamatsu, Kagawa 761-0396, Japan

March 31, 2006
June 2, 2006
December 20, 2006
phase contrast, single living cells, computed tomography, optical tweezers

We proposed using spectroscopy-tomography of single cells as a diagnostic tool for improving early cancer detection and treatment. This technology potentially obtains the 3D distribution of cellular components at high spatial resolution. We used spectroscopy-tomography to generate and analyze a series of cross-sectional images of a borosilicate glass microsphere 10µm in diameter, easily determining the distribution of internal submicron defects in the microsphere. We also used this to estimate the 3-dimensional refractive index distribution in a single human breast cancer cell. The nucleus, which has a high refractive index, is clearly distinguishable.

Cite this article as:
Yusuke Inoue, Ichirou Ishimaru, Toshiki Yasokawa,
Katsumi Ishizaki, , and Makoto Yoshida, “Novel Computed Tomography for Reconstructing Three-Dimensional Phase Contrast Images of Single Living Cells,” J. Robot. Mechatron., Vol.18, No.6, pp. 827-830, 2006.
Data files:
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  5. [5] H. Kobayashi, I. Ishimaru, G. Hashiguchi, F. Oohira, S. Kaio, and A. Hayashi, “A Proposal of Variable Phase-Contrast Microscope for Nano Geometry Measurement,” Proc. of ASME 2002 Japan-USA Symposium on Flexible Automation (2002JUSFA), Vol.III, pp. 1317-1320, 2002.
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Last updated on Mar. 05, 2021