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JRM Vol.18 No.6 pp. 827-830
doi: 10.20965/jrm.2006.p0827
(2006)

Letter:

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Novel Computed Tomography for Reconstructing Three-Dimensional Phase Contrast Images of Single Living Cells

Yusuke Inoue*, Ichirou Ishimaru**, Toshiki Yasokawa**,
Katsumi Ishizaki**, and Makoto Yoshida**

*Fuji Xerox Corporation, 2-17-22 Akasaka, Minato-ku, Tokyo 107-0052, Japan

**Faculty of Engineering, Kagawa University, 2217-20 Hayashi-cho, Takamatsu, Kagawa 761-0396, Japan

Received:
March 31, 2006
Accepted:
June 2, 2006
Published:
December 20, 2006
Creative Commons License
Keywords:
phase contrast, single living cells, computed tomography, optical tweezers
Abstract
We proposed using spectroscopy-tomography of single cells as a diagnostic tool for improving early cancer detection and treatment. This technology potentially obtains the 3D distribution of cellular components at high spatial resolution. We used spectroscopy-tomography to generate and analyze a series of cross-sectional images of a borosilicate glass microsphere 10µm in diameter, easily determining the distribution of internal submicron defects in the microsphere. We also used this to estimate the 3-dimensional refractive index distribution in a single human breast cancer cell. The nucleus, which has a high refractive index, is clearly distinguishable.
Cite this article as:
Y. Inoue, I. Ishimaru, T. Yasokawa, K. Ishizaki, and M. Yoshida, “Novel Computed Tomography for Reconstructing Three-Dimensional Phase Contrast Images of Single Living Cells,” J. Robot. Mechatron., Vol.18 No.6, pp. 827-830, 2006.
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References
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