Microfabrication and Laser Manipulation of Functional Microtool Using In-Situ Photofabrication
Hisataka Maruyama, Fumihito Arai, and Toshio Fukuda
Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
Single cell experiments have become very important for investigating unknown cell properties. We developed a novel technique to study individual cell properties on a chip using newly developed cell manipulation by laser tweezers with the photo-crosslinkable resin, using this resin to developed functional colored, fluorescent, cell binding, rotation free, and rope shaped microtools on a chip. Colored and fluorescent microtools are for cell manipulation using inexpensive image processing. Cell binding microtools are for high-speed transport of target cells. Rotation free microtools are for attitude control and precise force measurement of cells and DNA. Rope shaped microtools are for versatile manipulation. Laser tweezers is used to position-control microtools. We used a mercury lamp for UV illumination at the local area for combining microtools and fabricated our functional microtools and manipulated cells on the microchip.
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