Separation of Target Microbe by Laser Manipulation and Flow Control
Fumihito Arai*, Akihiko Ichikawa*, Toshio Fukuda*, Koji Horio**, Kouichi Itoigawa*** and Keisuke Morishima****
*Department of Micro System Engineering at Nagoya University, 1 Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
**Precursory Research for Embryonic Science and Technology, Japan Science and Technology Corporation Moritex Corporation, 1-3-3, Azamino-minami, Aoba-ku, Yokohama-city, Kanagawa prefecture 225-0012 Japan
***Tokai Rika Co., Ltd. Oguchi-cho, Niwa-gun, Aichi-ken 480-0195, Japan
****Kanagawa Academy of Science and Technology, KSP East 307, 3-2-1 Sakado, Takatsu-ku, Kawasaki, 213-0012, Japan
We developed random separation of single microorganisms, such as living cells and microbes, in a microfluid device under microscopy using laser manipulation and microchannel flow. The main flow in the microchannel splits at the exit of the sample chamber. One flow goes to the extraction port with high speed to extract the target. Another flow goes to the drain port. The target was transported by laser manipulation from the sample chamber to put on the main flow. Since the force balance of the laser-trapped object is important for stable manipulation of the target, we propose separation point control around the sample chamber exit. Pressure balance was adjusted and the separation point was controlled. Some preliminary experiments are conducted to show effectiveness. The target was trapped by the laser, transported to the main flow around the split area through stagnation, and was extracted from the extraction port. Separation time was reduced less than 10 seconds and the success of separation improved 80%.
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