IJAT Vol.11 No.6 pp. 941-946
doi: 10.20965/ijat.2017.p0941


Transfer-Print of CNTs and its Application to Cell Scaffold

Arata Kaneko, Yuuki Miyazaki, and Tatsuya Goto

Faculty of System Design, Tokyo Metropolitan University
6-6 Asahigaoka, Hino, Tokyo 191-0065, Japan

Corresponding author

February 15, 2017
April 17, 2017
Online released:
October 31, 2017
November 5, 2017
carbon nanotube, CNTs, transfer-print, wettability, scaffold, cell

A bio-chip using cultured cells is developed for an application to drug screening. Carbon nanotubes (CNTs) are a candidate for this electrode material. A transfer-prints is expected to be a CNT-patterning technique applicable to soft material. This present paper is intended to show some basic properties about the transfer-print of CNTs, and also to demonstrate the possibility of the CNTs as a cell scaffold. The present study prepared several types of surface-modified Si substrate with different wettability to investigate the effects of wettability on the transferring ratio of CNTs. Some Si substrates are terminated by OH or H groups, while other substrates are coated with hydrophobic or hydrophilic self-assembled monolayers. The stamps for transfer-print, which have circular dots (50-μm diameter) or a straight ridge (50-μm width) array, are fabricated using poly-dimethyl-siloxane (PDMS). The surfaces of PDMS stamps are inked by single-walled CNTs by a pre-transferring or casting process. The transfer-prints to surface-modified Si surfaces allow the CNTs to be formed in lines of several tens of micrometers, while the coverage of transfer-printed CNTs is also dominated by surface wettability. The coverage of transfer-printed CNTs increases with the water contact angle of the Si surface. It is reasonable that the transfer-print of CNTs is performed by hydrophobic interactions. Meanwhile, two kinds of polymer (polystyrene (PS) and polyethylene terephthalate (PET)) sheets are also utilized as a substrate. The transfer-prints with heating around the softening point of the polymer allow CNTs to be accurately patterned into an array of 50-μm dots. The coverage of CNTs is 94% on the PET substrate. The PS sheet with patterned CNTs is applied to a cell scaffold. PC12 cells are cultured on the PS sheets so that the cells are selectively adhered to the transfer-printed CNTs. The adhered cells are extended with some pseudopods. It is demonstrated that the transfer-printed CNTs are expected to be electrodes of the cell scaffold.

Cite this article as:
A. Kaneko, Y. Miyazaki, and T. Goto, “Transfer-Print of CNTs and its Application to Cell Scaffold,” Int. J. Automation Technol., Vol.11, No.6, pp. 941-946, 2017.
Data files:
  1. [1] A. Béduer, C. Vieu, F. Arnauduc, J. C. Sol, I. Loubinoux, and L. Vaysse, “Engineering of adult human neural stem cells differentiation through surface micropatterning,” Biomaterials, Vol.33, pp. 504-514, 2012.
  2. [2] J. C. Chang, G. J. Brewer, and B. C. Wheeler, “A modified microstamping technique enhances polylysine transfer and neuronal cell patterning,” Biomaterials, Vol.24, pp. 2863-2870, 2003.
  3. [3] I. Takeda, M. Kawanabe, and A. Kaneko, “Autonomous Patterning of Cells on Microstructured Fine Particles,” Materials Science and Engineering C , Vol.50, pp. 173-178, 2015.
  4. [4] I. Takeda, M. Kawanabe, and A. Kaneko, “An investigation of cell adhesion and growth on micro/nano-scale structured surface -Self-assembled micro particles as a scaffold,” Precision Engineering, Vol.43, pp. 294-298, 2016.
  5. [5] N. Moronuki, “Functional Texture Design and Texturing Processes,” Int. J. of Automation Technology, Vol.10, No.1, pp. 4-15, 2016.
  6. [6] H. Fujie, K. Oya, Y. Tani, K. Suzuki, and N. Nakamura, “Stem Cell-Based Self-Assembled Tissues Cultured on a Nano-Periodic-Structured Surface Patterned Using Femtosecond Laser Processing,” Int. J. of Automation Technology, Vol.10, No.1, pp. 55-61, 2016.
  7. [7] A. Kaneko and I. Takeda, “Textured Surface of Self-Assembled Particles as a Scaffold for Selective Cell Adhesion and Growth,” Int. J. of Automation Technology, Vol.10, No.1, pp. 62-68, 2016.
  8. [8] K. Yamada, T. Ueda, A. Hosokawa, T. Furumoto, and R. Tanaka, “Dental Treatment with Laser Beam – Monitoring Enabling Safe Hard-Tooth-Tissue Removal –,” Int. J. of Automation Technology, Vol.3, No.5, pp. 494-501, 2009.
  9. [9] C. Giada, M. T. Francesca, K. V. Zeynep, L. Jummi, V. Ambra, Q. Mildred, C. Sara, P. Maurizio, and B. Laura, “Carbon Nanotube Scaffolds Tune Synaptic Strength in cultured Neuronal Circuits: Novel Frontiers in Nanomaterial-Tissue Interactions,” J. of Neuroscience, Vol.36, pp. 12945-12953, 2011.
  10. [10] A. Greenbauma, S. Anava, A. Ayali, M. Sheina, M. David-Pura, E. Ben-Jacobc, and Y. Haneina, “One-to-one neuron-electrode interfacing,” J. of Neuroscience Methods, Vol.182, pp. 219-224, 2009.
  11. [11] A. Kaneko, H. Murakami, and T. Yamashita, “Effect of Surface Property on Transfer-Print of Au Thin-Film to Micro-Structured Substrate,” Int. J. of Automation Technology, Vol.9, No.4, pp. 411-417, 2015.
  12. [12] M. A. Meitl, Y. Zhou, A. Gaur, S. Jeon, M. L. Usrey, M. S. Strano, and J. A. Rogers, “Solution Casting and Transfer Printing Single-Walled Carbon Nanotube Films,” Nano Letters, Vol.4, No.9, pp. 1643-1647, 2009.
  13. [13] S. Pei, J. Du, Y. Zeng, C. Liu, and H. M. Cheng, “The fabrication of a carbon nanotube transparent conductive film by electrophoretic deposition and hot-pressing transfer,” Nanotechnology, Vol.20, p. 235707, 2009.
  14. [14] V. K. Sangwan, V. W. Ballarotto, D. R. Hines, M. S. Fuhrer, and E. D. Williams, “Controlled growth, patterning and placement of carbon nanotube thin films,” Solid-State Electronics, Vol.54, pp. 1204-1210, 2010.
  15. [15] Y. Zhou, L. Hu, and G. Gruner, “A method of printing carbon nanotube thin films,” Applied Physics Letters, Vol.88, pp. 123-109, 2006.
  16. [16] D. Zhang, K. Ryu, X. Liu, E. Polikarpov, J. Ly, M. E. Tompson, and C. Zhou, “Transparent, Conductive, and Flexible Carbon Nanotube Films and Their Application in Organic Light-Emitting Diodes,” Nanoletters, Vol.6, No.9, pp. 1880-1886, 2006.
  17. [17] A. Béduer, F. Seichepine, E. Flahaut, and C. Vieu, “A simple and versatile micro contact printing method for generating carbon nanotubes patterns on various substrates,” Microelectronic Engineering, Vol.97, pp. 301-305, 2012.
  18. [18] K. Fuchsberger, A. L. Goff, F. M. Toma, A. Goldoni, M. Giugliano, M. Stelzle, and M. Prato, “Multiwalled Carbon-Nanotube-Functionalized Microelectrode Arrays Fabricated by Microcontact Printing: Platform for Studying Chemical and Electrical Neuronal Signaling,” Small, Vol.7, No.4, pp. 524-530, 2011.
  19. [19] Z. Wu, Z. Chen, X. Du, J. M. Logan, J. Sippel, M. Nikolou, K. Kamaras, J. R. Reynolds, D. B. Tanner, A. F. Hebard, and A. G. Rinzler, “Transparent, Conductive Carbon Nanotube Films,” Science, Vol.305, No.5688, pp. 1273-1276, 2004.
  20. [20] J. N. Israelachvili, “Intermolecular and Surface Forces,” Nanotechnology, Elsevier Inc., USA, 2004.
  21. [21] N. Tanaka, “Solubility Parameter Prediction of Polymers by Thermal Analysis,” Netsu Sokutei, Vol.30 No.3, pp. 125-130, 2003.
  22. [22] K. Hiratsuka, A. Bohno, and H. Endo, “Water droplet lubrication between hydrophilic and hydrophobic surfaces,” J. of Physics: Conf. Series, Vol.89, Issue 1, 012012, 2007.
  23. [23] P. Potejanasak, M. Yoshino, M. Terano, and M. Mita, “Efficient Fabrication Process of Metal Nanodot Arrays Using Direct Nanoimprinting Method with a Polymer Mold,” Int. J. of Automation Technology, Vol.9, No.6, pp. 629-635, 2015.

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Last updated on Dec. 11, 2018