Textured Surface of Self-Assembled Particles as a Scaffold for Selective Cell Adhesion and Growth
Arata Kaneko and Iwori Takeda
Faculty of System Design, Tokyo Metropolitan University
6-6 Asahigaoka, Hino, Tokyo 191-0065, Japan
SiO2 particles (φ 1 μm) self-assemble into hexagonal arrangements on a glass substrate. Dip-coating is also used to produce linear patterns of particles several tens of micrometers in width on substrates patterned with octadecyltrichlorosilane (OTS). Some particles are coated with specific proteins via electrochemical adsorption and structured on a glass substrate. The upper surfaces of self-assembled particles have specifically-ordered asperities that can be called textures. These textured surfaces are applied to a cell scaffold. PC12 and HeLa cells adhere to the textured surfaces of particles more often than they adhere to flat (smooth) surfaces. The cells are located on approximately 50-μm-width of self-assembled particles. Thus, it is found that the textured surface of particles functions as a template for autonomous cell patterning. An in-situ observation shows that the selective adhesion of cells is achieved by their extensions and migrations from the flat region to the particles. Coating particles with proteins enhances cell adhesiveness in such a way that isolated cells adhere to the linear patterns of particles in straight lines. The textured surfaces of particles also affect cell growth. As cell growth is restricted on the textured surfaces of particles, a confluent state of aggregated cells is achieved on only a linear pattern of particles.
-  Z. L. Zhang, T. Asano, H. Uno, R. Tero, M. Suzui, S. Nakao, T. Kaito, K. Shibasaki, M. Tominaga, Y. Utsumi, Y. L. Gao, and T. Urisu, “Fabrication of Si-based planar type patch clamp biosensor using silicon on insulator substrate,” Thin Solid Films, Vol.516, pp. 2813-2815, 2008.
-  M. Xue, S. Guo, X. S. Zhao, and T. Cao, “Fabrication of ultrafine protein arrays on easy-fabricated metallic nanostructures,” Scripta Materials, Vol.58, pp. 854-857, 2008.
-  C. H. Jang, M. L. Tingey, N. L. Korpi, G. J. Wiepz, J. H. Schiller, P. J. Bertics, and N. L. Abbott, “Using Liquid Crystals to Report Membrane Proteins Captured by Affinity Microcontact Printing from Cell Lysates and Membrane Extracts,” J. Am. Chem. Soc., Vol.127, No.25, pp. 8912-8913, 2005.
-  D. Kleinfeld, K. H. Kahler, and P. E. Hockberger, “Controlled outgrowth of dissociated neurons on patterned substrates,” J. Neurosci., Vol.8, pp. 4098-4120, 1988.
-  A. Ranella, M. Barberoglou, S. Bakogianni, C. Fotakis, and E. Stratakis, “Tuning cell adhesion by controlling the roughness and wettability of 3D micro/nano silicon structures,” Acta Biomater., Vol.6, pp. 2711-2720, 2010.
-  J. M. Karpa, Y. Yeo. W. Gerg, C. Cannizarro, K. Yan, D. S. Kohane, G. Vunjak-Novakovic, R. S. Langer, and M. Radisic, “A photolithographic method to create cellular micropatterns,” Biomaterials, Vol.27, No.27, pp. 4755-4764, 2006.
-  J. Ahn, S. J. Son, and J. Min, “The control of cell adhesion on a PMMA polymer surface consisting of nanopillar arrays,” J. Biotechnol., Vol.164, pp. 543-548, 2013.
-  S. Nomura, H. Kojima, Y. Ohyabu, K. Kuwabara1, A. Miyauchi, and T. Uemura, “Cell culture on nanopillar sheet: Study of HeLa cells on Nanopillar sheet,” Jpn. J. Appl. Phys., Vol.44, L1184-1186, 2005.
-  S. Yamamoto, M. Tanaka, H. Sunami, E. Ito, S. Yamashita, Y. Morita, and M. Shimomura, “Effect of Honeycomb-Patterned Surface Topography on the Adhesion and Signal Transduction of Porcine Aortic Endothelial Cells,” Langmuir, Vol.23 No.15, pp. 8114-8120, 2007.
-  A. Kaneko, D. Aruga, N. Moronuki, and Y. Kanamori, “Self-assembly of Surface-modified Fine Particles on Patterned Substrate,” Proc. 9th euspen, Vol.2, pp. 414-417, 2009.
-  Y. Kanamori, A. Kaneko, N. Moronuki, and T. Kubo, “Self-assembly of fine particles on patterned wettability in dip coating and its scale extension with contact printing,” J. Adv. Mech. Des. Syst., Vol.2, pp. 783-791, 2008.
-  M. Nishio, N. Moronuki, and A. Kaneko, “Instability Phenomenon in Dip-Coating Process for Self-Assembly of Fine Particles and Design Countermeasures,” Int. J. of Automation Technology, Vol.5, No.5, pp. 688-693, 2011.
-  I. Takeda, M. Kawanabe, and A. Kaneko, “Autonomous patterning of cells on microstructured fine particles,” Material Science and Engineering C, Vol.50, pp. 173-178, 2015.
-  I. Takeda, S. Serizawa, and A. Kaneko, “An Investigation of cell adhesion and growth on micro/nano-scale structured surface,” Precision Engineering, 2015 (to be published).
-  A. Kaneko, T. Sugihara, H. Murakami, I. Takeda, Y. Tanaka, and N. Moronuki, “Fabrication of spatially-patterned cells using selective adhesion on pre-structured fine particles,” Key Engineering Materials, Vol.523-524, pp. 615-620, 2012.
-  R. Dahint, E. Trileva, H. Acunman, U. Konrad, M. Zimmer, V. Stadler, and M. Himmelhaus, “Optically responsive nanoparticle layers for the label-free analysis of biospecific interactions in array format,” Biosensors and Bioelectronics, Vol.22, pp. 3174-3181, 2007.
-  S. Fukuzaki, H. Urano, and K. Nagata, “Adsorption of bovine serum albumin onto metal oxide surfaces,” J. Ferment. Bioeng., Vol.81, pp. 163-167, 1996.
-  I. Takeda, M. Kawanabe, and A. Kaneko, “An investigation of cell adhesion and growth on micro/nano-scale structured surface,” Precision Engineering, 2015 (to be published).
-  T. Hirono, K. Torimitsu, A. Kawana, and J. Fukuda, “Recognition of artificial microstructures by sensory nerve fibers in culture,” Brain Res., Vol.446, No.1, pp. 189-194, 1988.
-  K. Torimitsu and A. Kawana, “Selective growth of sensory nerve fibers on metal oxide pattern in culture,” Dev. Brain Res., Vol.51, No.1, pp. 128-131, 1990.
This article is published under a Creative Commons Attribution-NoDerivatives 4.0 International License.