This paper describes a visual servosystem that observes a moving microorganism within the optical microscope view field. The visual servosystem consists of an optical microscope, a high-speed CCD camera, a controller, and XY stages. The camera takes an image every 4 milliseconds. The target’s center of gravity is calculated using inverse white-to-black processing and blocking processing. Its position is fed back to XY stages so that the target reaches the center of the view field. The control program is from LabVIEW. As a result, by means of this visual servosystem, the image of a very small object, for example, a kind of lobster of a size of 0.4 ∼ 0.6 mm which moves in the water at the maximum speed of 4 mm/s can be kept in view based on a method whereby, on the XY stages on which the moving object can continue to be observed.

1. [1] Y. Nakabo, M. Ishikawa, H. Toyoda, and S. Mizuno, “1ms Column Parallel Vision System and Its Application of High Speed Target Tracking,” Proc. of IEEE Int. Conf. on Robotics and Automation, pp. 650-655, 2000.
2. [2] H. Oku, M. Ishikawa, Theodorus, and K. Hashimoto, “High-speed autofocusing of a cell using diffraction pattern,”Optics Express, Vol.14, Issue 9, pp. 3952-3960, 2006.
3. [3] H. Oku, N. Ogawa, K. Shiba, M. Yoshida, and M. Ishikawa, “How to Track Spermatozoa using High-Speed Visual Feedback,” Proc. of 30th Annual Int. Conf. of the IEEE Engineering in Medicine and Biology Society, pp. 125-128, 2008.
4. [4] H. Toyoda, N. Sakisaka, S. Mizuno, Y. Nakabo, and M. Ishikawa, “Ultra-high speedy Intelligent System CPV,” Proc. of 6th Image Sensing Symposium, pp. 213-216, 2000.
5. [5] H. Fujioka and K. Nakamae, “Fundamental Image Processing,” Shokodo, p. 3, 2002 (in Japanese).