Three-dimensional multiple fish tracking has gained significant research interest in quantifying fish behavior. However, most tracking techniques use a high frame rate, which is currently not viable for real-time tracking applications. This study discusses multiple fish-tracking techniques using low-frame-rate sampling of stereo video clips. The fish were tagged and tracked based on the absolute error of the predicted indices using past and present fish centroid locations and a deterministic frame index. In the predictor sub-system, linear regression and machine learning algorithms intended for nonlinear systems, such as the adaptive neuro-fuzzy inference system (ANFIS), symbolic regression, and Gaussian process regression (GPR), were investigated. The results showed that, in the context of tagging and tracking accuracy, the symbolic regression attained the best performance, followed by the GPR, that is, 74% to 100% and 81% to 91%, respectively. Considering the computation time, symbolic regression resulted in the highest computing lag of approximately 946 ms per iteration, whereas GPR achieved the lowest computing time of 39 ms.

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