Top > JRM > Image-Based Gel Encapsulation of Suspended Single Cells for Pa ...

single-rb.php

JRM Vol.35 No.5 pp. 1177-1184
doi: 10.20965/jrm.2023.p1177
(2023)

Paper:

Views over last 60 days: 337

Image-Based Gel Encapsulation of Suspended Single Cells for Parallel Single-Cell Screening

Venkatesh Kumar Panneer Selvam*, Muhammad Luqman Arief Bin Kamaludin*, Ghulam Murtaza*, Rifat Hussain Chowdhury*, Tanmay Debnath*, Shunya Okamoto*, Takayuki Shibata* ORCID Icon, Tuhin Subhra Santra** ORCID Icon, and Moeto Nagai*,*** ORCID Icon

*Department of Mechanical Engineering, Toyohashi University of Technology
1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan

**Department of Engineering Design, Indian Institute of Technology
Chennai, Tamil Nadu 600, India

***Institute for Research on Next-generation Semiconductor and Sensing Science, Toyohashi University of Technology
1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan

Received:
April 20, 2023
Accepted:
August 18, 2023
Published:
October 20, 2023
Creative Commons License
Keywords:
single-cell screening, image processing, multi-light irradiation, photo-crosslinkable gel, cell encapsulation
Abstract

Single-cell screening, which has revolutionized the life sciences, is an important method for detecting, separating, or treating specific cells based on desired characteristics. Previously, single cells of interest were manually identified in an image, which required human labor and time. We developed an automated photopolymerization system to encapsulate suspended single cells in approximately 50-µm photo-crosslinkable hydrogel squares. An image was captured, and single cells were selected from grouped cells based on image processing. A generated image was transferred to a digital micromirror device (DMD), and in parallel, target-suspended single cells were encapsulated in gelatin methacryloyl (GelMA) hydrogels. We built a data transfer platform based on a Power Automate Desktop (PAD), completed the data transfer, and projected the processed image onto a sample in 10 s, ensuring a minimum alignment error of 6.2 µm.

Image-based gel encapsulation of suspended single cells

Image-based gel encapsulation of suspended single cells

Cite this article as:
V. Selvam, M. Kamaludin, G. Murtaza, R. Chowdhury, T. Debnath, S. Okamoto, T. Shibata, T. Santra, and M. Nagai, “Image-Based Gel Encapsulation of Suspended Single Cells for Parallel Single-Cell Screening,” J. Robot. Mechatron., Vol.35 No.5, pp. 1177-1184, 2023.
Data files:
References
  1. [1] L. Valihrach, P. Androvic, and M. Kubista, “Platforms for single-cell collection and analysis,” Int. J. of Molecular Sciences, Vol.19, No.3, Article No.807, 2018. https://doi.org/10.3390/ijms19030807
  2. [2] A. Dalili, E. Samiei, and M. Hoorfar, “A review of sorting, separation and isolation of cells and microbeads for biomedical applications: Microfluidic approaches,” Analyst, Vol.144, No.1, pp. 87-113, 2019. https://doi.org/10.1039/C8AN01061G
  3. [3] V. Fitzgerald and P. Leonard, “Single cell screening approaches for antibody discovery,” Methods, Vol.116, pp. 34-42, 2017. https://doi.org/10.1016/j.ymeth.2016.11.006
  4. [4] N. B. Ben-Moshe et al., “Predicting bacterial infection outcomes using single cell RNA-sequencing analysis of human immune cells,” Nature Communications, Vol.10, No.1, Article No.3266, 2019. https://doi.org/10.1038/s41467-019-11257-y
  5. [5] C. Kim et al., “Chemoresistance evolution in triple-negative breast cancer delineated by single-cell sequencing,” Cell, Vol.173, No.4, pp. 879-893.e13, 2018. https://doi.org/10.1016/j.cell.2018.03.041
  6. [6] A. K. Mitra et al., “Single-cell analysis of targeted transcriptome predicts drug sensitivity of single cells within human myeloma tumors,” Leukemia, Vol.30, No.5, pp. 1094-1102, 2016. https://doi.org/10.1038/leu.2015.361
  7. [7] B. E. Debs, R. Utharala, I. V. Balyasnikova, A. D. Griffiths, and C. A. Merten, “Functional single-cell hybridoma screening using droplet-based microfluidics,” Proc. of the National Academy of Sciences, Vol.109, No.29, pp. 11570-11575, 2012. https://doi.org/10.1073/pnas.1204514109
  8. [8] C. Krieg et al., “High-dimensional single-cell analysis predicts response to anti-PD-1 immunotherapy,” Nature Medicine, Vol.24, No.2, pp. 144-153, 2018. https://doi.org/10.1038/nm.4466
  9. [9] M. B. Dainiak, A. Kumar, I. Y. Galaev, and B. Mattiasson, “Methods in cell separations,” A. Kumar, I. Y. Galaev, and B. Mattiasson (Eds.), “Cell separation: Fundamentals, analytical and preparative methods,” Springer Berlin Heidelberg, pp. 1-18, 2007.
  10. [10] P. Shinde et al., “Current trends of microfluidic single-cell technologies,” Int. J. of Molecular Sciences, Vol.19, No.10, Article No.3143, 2018. https://doi.org/10.3390/ijms19103143
  11. [11] X. Liao, M. Makris, and X. M. Luo, “Fluorescence-activated cell sorting for purification of plasmacytoid dendritic cells from the mouse bone marrow,” J. of Visualized Experiments, No.117, Article No.54641, 2016. https://doi.org/10.3791/54641
  12. [12] A. Blasius, W. Vermi, A. Krug, F. Facchetti, M. Cella, and M. Colonna, “A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha,” Blood, Vol.103, No.11, pp. 4201-4206, 2004. https://doi.org/10.1182/blood-2003-09-3108
  13. [13] A. Gross, J. Schoendube, S. Zimmermann, M. Steeb, R. Zengerle, and P. Koltay, “Technologies for single-cell isolation,” Int. J. of Molecular Sciences, Vol.16, No.8, pp. 16897-16919, 2015. https://doi.org/10.3390/ijms160816897
  14. [14] G. Welzel, D. Seitz, and S. Schuster, “Magnetic-activated cell sorting (MACS) can be used as a large-scale method for establishing zebrafish neuronal cell cultures,” Scientific Reports, Vol.5, No.1, Article No.7959, 2015. https://doi.org/10.1038/srep07959
  15. [15] H. Valli et al., “Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells,” Fertility and Sterility, Vol.102, No.2, pp. 566-580.e7, 2014. https://doi.org/10.1016/j.fertnstert.2014.04.036
  16. [16] S. Ota et al., “Ghost cytometry,” Science, Vol.360, No.6394, pp. 1246-1251, 2018. https://doi.org/10.1126/science.aan0096
  17. [17] N. Nitta et al., “Intelligent image-activated cell sorting,” Cell, Vol.175, No.1, pp. 266-276.e13, 2018. https://doi.org/10.1016/j.cell.2018.08.028
  18. [18] C. A. LaBelle, A. Massaro, B. Cortés-Llanos, C. E. Sims, and N. L. Allbritton, “Image-based live cell sorting,” Trends in Biotechnology, Vol.39, No.6, pp. 613-623, 2021. https://doi.org/10.1016/j.tibtech.2020.10.006
  19. [19] B. Cortés-Llanos, Y. Wang, C. E. Sims, and N. L. Allbritton, “A technology of a different sort: Microraft arrays,” Lab on a Chip, Vol.21, No.17, pp. 3204-3218, 2021. https://doi.org/10.1039/D1LC00506E
  20. [20] J. Jin et al., “Robotic data acquisition with deep learning enables cell image-based prediction of transcriptomic phenotypes,” Proc. of the National Academy of Sciences, Vol.120, No.1, Article No.e2210283120, 2022. https://doi.org/10.1073/pnas.2210283120
  21. [21] M. Nagai, K. Kato, K. Oohara, and T. Shibata, “Pick-and-place operation of single cell using optical and electrical measurements for robust manipulation,” Micromachines, Vol.8, No.12, Article No.350, 2017. https://doi.org/10.3390/mi8120350
  22. [22] Y. Yamanishi, T. Nakano, Y. Sawada, K. Itoga, T. Okano, and F. Arai, “Maskless gray scale lithography and its 3d microfluidic applications,” J. Robot. Mechatron., Vol.23, No.3, pp. 426-433, 2011. https://doi.org/10.20965/jrm.2011.p0426
  23. [23] M. Nagai, K. Oohara, K. Kato, T. Kawashima, and T. Shibata, “Development and characterization of hollow microprobe array as a potential tool for versatile and massively parallel manipulation of single cells,” Biomedical Microdevices, Vol.17, No.2, Article No.41, 2015. https://doi.org/10.1007/s10544-015-9943-z
  24. [24] M. Nagai, K. Kato, S. Soga, T. S. Santra, and T. Shibata, “Scalable parallel manipulation of single cells using micronozzle array integrated with bidirectional electrokinetic pumps,” Micromachines, Vol.11, No.4, Article No.442, 2020. https://doi.org/10.3390/mi11040442
  25. [25] R. Negishi et al., “Gel-based cell manipulation method for isolation and genotyping of single-adherent cells,” Analyst, Vol.144, No.3, pp. 990-996, 2019. https://doi.org/10.1039/C8AN01456F
  26. [26] T. Yoshino, T. Tanaka, S. Nakamura, R. Negishi, M. Hosokawa, and T. Matsunaga, “Manipulation of a single circulating tumor cell using visualization of hydrogel encapsulation toward single-cell whole-genome amplification,” Analytical Chemistry, Vol.88, No.14, pp. 7230-7237, 2016. https://doi.org/10.1021/acs.analchem.6b01475
  27. [27] R. Negishi, K. Takai, T. Tanaka, T. Matsunaga, and T. Yoshino, “High-throughput manipulation of circulating tumor cells using a multiple single-cell encapsulation system with a digital micromirror device,” Analytical Chemistry, Vol.90, No.16, pp. 9734-9741, 2018. https://doi.org/10.1021/acs.analchem.8b00896
  28. [28] M. B. Browning, S. N. Cereceres, P. T. Luong, and E. M. Cosgriff-Hernandez, “Determination of the in vivo degradation mechanism of PEGDA hydrogels,” J. of Biomedical Materials Research Part A, Vol.102, No.12, pp. 4244-4251, 2014. https://doi.org/10.1002/jbm.a.35096
  29. [29] M. Zhu, Y. Wang, G. Ferracci, J. Zheng, N.-J. Cho, and B. H. Lee, “Gelatin methacryloyl and its hydrogels with an exceptional degree of controllability and batch-to-batch consistency,” Scientific Reports, Vol.9, No.1, Article No.6863, 2019. https://doi.org/10.1038/s41598-019-42186-x
  30. [30] M. Nagai et al., “Parallel photothermal coalescence of biocompatible photocurable PEGDA droplets,” IEEJ Trans. on Sensors and Micromachines, Vol.143, No.3, pp. 49-54, 2023. https://doi.org/10.1541/ieejsmas.143.49
  31. [31] T. Debnath, R. Hattori, S. Okamoto, T. Shibata, T. S. Santra, and M. Nagai, “Automated detection of patterned single-cells within hydrogel using deep learning,” Scientific Reports, Vol.12, No.1, Article No.18343, 2022. https://doi.org/10.1038/s41598-022-22774-0

Creative Commons License  This article is published under a Creative Commons Attribution-NoDerivatives 4.0 Internationa License.

*This site is desgined based on HTML5 and CSS3 for modern browsers, e.g. Chrome, Firefox, Safari, Edge, Opera.

Last updated on Dec. 06, 2024