JRM Vol.35 No.5 pp. 1177-1184
doi: 10.20965/jrm.2023.p1177


Image-Based Gel Encapsulation of Suspended Single Cells for Parallel Single-Cell Screening

Venkatesh Kumar Panneer Selvam*, Muhammad Luqman Arief Bin Kamaludin*, Ghulam Murtaza*, Rifat Hussain Chowdhury*, Tanmay Debnath*, Shunya Okamoto*, Takayuki Shibata* ORCID Icon, Tuhin Subhra Santra** ORCID Icon, and Moeto Nagai*,*** ORCID Icon

*Department of Mechanical Engineering, Toyohashi University of Technology
1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan

**Department of Engineering Design, Indian Institute of Technology
Chennai, Tamil Nadu 600, India

***Institute for Research on Next-generation Semiconductor and Sensing Science, Toyohashi University of Technology
1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan

April 20, 2023
August 18, 2023
October 20, 2023
single-cell screening, image processing, multi-light irradiation, photo-crosslinkable gel, cell encapsulation

Single-cell screening, which has revolutionized the life sciences, is an important method for detecting, separating, or treating specific cells based on desired characteristics. Previously, single cells of interest were manually identified in an image, which required human labor and time. We developed an automated photopolymerization system to encapsulate suspended single cells in approximately 50-µm photo-crosslinkable hydrogel squares. An image was captured, and single cells were selected from grouped cells based on image processing. A generated image was transferred to a digital micromirror device (DMD), and in parallel, target-suspended single cells were encapsulated in gelatin methacryloyl (GelMA) hydrogels. We built a data transfer platform based on a Power Automate Desktop (PAD), completed the data transfer, and projected the processed image onto a sample in 10 s, ensuring a minimum alignment error of 6.2 µm.

Image-based gel encapsulation of suspended single cells

Image-based gel encapsulation of suspended single cells

Cite this article as:
V. Selvam, M. Kamaludin, G. Murtaza, R. Chowdhury, T. Debnath, S. Okamoto, T. Shibata, T. Santra, and M. Nagai, “Image-Based Gel Encapsulation of Suspended Single Cells for Parallel Single-Cell Screening,” J. Robot. Mechatron., Vol.35 No.5, pp. 1177-1184, 2023.
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Last updated on May. 10, 2024