JRM Vol.25 No.4 pp. 611-618
doi: 10.20965/jrm.2013.p0611


A Bio-Manipulation Method Based on the Hydrodynamic Force of Multiple Microfluidic Streams

Yaxiaer Yalikun, Yoshitake Akiyama, Takayuki Hoshino,
and Keisuke Morishima

Graduate School of Engineering, Department of Mechanical Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan

February 22, 2013
June 3, 2013
August 20, 2013
hydrodynamic forces, microfluidic stream, open space, noncontact bio-manipulation, multi-scale manipulation

This paper proposes a multiple microfluidic streambased manipulation (MMSM) system for bio-objects. It uses micro hydrodynamics and lab on chip (LOC) technology. Our method can implement the functions of micro manipulation and micro assembly of bio-objects in an open space without contact. Compared to other conventional bio-micro-manipulation and assembly methods, this system manipulates micro objects by controlling multiple microfluidic streams onto them from various directions. The advantages of this method are that it performs open space, multifunction, multi-scale, multi-degree-of-freedom, and non-invasive 3D manipulation. These microfluidic streams are generated simultaneously from multiple orifices. By regulating the parameters of the microfluidic stream, such as the position and number of operating orifices and the flow rate, the direction and velocity of the object can be controlled. To verify this principle, we design an open-space fluidic system for on-chip manipulation and calculated velocity and direction of the microfluidic stream using CFD simulation. Then the prototype microchip with an array of nine orifices is fabricated from glass. In experiments, demonstrations of rectilinear motion of a single cell andmicro particle are observed. The results presented in this paper show that this MMSM is capable of biomicromanipulation and micro assembly of bio-objects.

Cite this article as:
Yaxiaer Yalikun, Yoshitake Akiyama, Takayuki Hoshino, and
and Keisuke Morishima, “A Bio-Manipulation Method Based on the Hydrodynamic Force of Multiple Microfluidic Streams,” J. Robot. Mechatron., Vol.25, No.4, pp. 611-618, 2013.
Data files:
  1. [1] T. Fukuda and F. Arai, “Prototyping design and automation of micro/nano manipulation system,” Proc. of IEEE Int. Conf. on Robotics and Automation, Vol.1, pp. 192-197, 2000.
  2. [2] B. Solano and D. Wood, “Design and testing of a polymeric micro gripper for cell manipulation. Microelectronic Engineering,” Microelectronic Engineering, Vol.84, pp. 1219-1222, 2007.
  3. [3] F. Beyeler, S. Member, A. Neild, S. Oberti, D. J. Bell, Y. Sun, and J. Dual, “Monolithically Fabricated Microgripper With Integrated Force Sensor for Manipulating Micro objects and Biological Cells Aligned in an Ultrasonic Field,” J. of Microelectromechanical Systems, Vol.16, No.1, pp. 7-15, 2007.
  4. [4] A. Ashkin, “Optical Trapping and Manipulation of Neutral Particles Using Lasers: A Reprint Volume With Commentaries,” World Scientific Publishing Company, 2006.
  5. [5] K. C. Neuman and S. M. Block, “Optical trapping,” Review of Scientific Instruments, Vol.75, pp. 2787-2809, 2004.
  6. [6] P. Y. Chiou, A. T. Ohta, and M. C. Wu, “Massively Parallel Manipulation of Single Cells and Micro particles Using Optical Images,” Nature, Vol.436, pp. 370-372, 2005.
  7. [7] A. E. Cohen, “Control of nanoparticles with arbitrary twodimensional force fields,” Physical review letters, Vol.94, p. 118102, 2005.
  8. [8] H.M. Hertz, “Standingwave acoustic trap for nonintrusive positioning of micro particles,” J. of Applied Physics, Vol.78, pp. 4845-4849, 1995.
  9. [9] S. Grilli and F. Pietro, “Electrophoretic trapping of suspended particles by selective pyroelectric effect in lithium niobate crystals,” Applied Physics Letters, Vol.92, pp. 232902-232902-3, 2008.
  10. [10] S. K. Srivastava, A. Gencoglu, and A. R. Minerick, “DC insulator dielectrophoretic applications in microdevice technology: a review,” Analytical and Bioanalytical Chemistry, Vol.399, pp. 301-321, 2010.
  11. [11] D. D. Carlo, Y. L. Wu, and P. L. Luke, “Dynamic Single Cell Culture Array,” Lab on a Chip, Vol.6, pp. 1445-1449, 2006.
  12. [12] B. R. Lutz, J. Chen, and D. T. Schwartz, “Hydrodynamic Tweezers: 1. Noncontact Trapping of Single Cells Using Steady Streaming Microeddies,” Analytical Chemistry, Vol.78, pp. 5429-5435, 2006.
  13. [13] M. Hagiwara, T. Kawahara, and F. Arai, “Local streamline generation by mechanical oscillation in a microfluidic chip for noncontact cell manipulations,” Applied Physics Letters, Vol.101, pp. 074102-074102-4, 2012.
  14. [14] M. Tanyeri, E. M. Johnson-Chavarria, and C. M. Schroeder, “Hydrodynamic trap for single particles and cells,” Applied Physics Letters, Vol.96, pp. 1786-1794, 2010.
  15. [15] Z. X. Liu, Z. Q. Chen, and M. H. Shi, “Thermophoresis of particles in aqueous solution in micro-channel,” Applied Thermal Engineering, Vol.29, No.5-6, pp. 1020-1025, 2009.
  16. [16] S. Iwaki, H. Morimasa, T. Noritsugu, and M. Kobayashi, “Contactless manipulation of an object on a plane surface using multiple air jets,” Proc. of IEEE Int. Conf. on Robotics and Automation, pp. 3257-3262, 2011.
  17. [17] J. P. Owen and W. S. Ryu, “The effects of linear and quadratic drag on falling spheres: an undergraduate laboratory,” European Journal of Physics, Vol.26, pp. 1085-1091, 2005.
  18. [18] R. Wayne and M. P. Staves, “The density of the cell sap and endoplasm of Nitellopsis and Chara,” Plant and Cell Physiology, Vol.32, No.8, pp. 1137-114, 1991.

  19. Supporting Online Materials:
  20. [a] [Accessed July 18, 2013]

*This site is desgined based on HTML5 and CSS3 for modern browsers, e.g. Chrome, Firefox, Safari, Edge, Opera.

Last updated on Feb. 25, 2021