Fluorescent-Based Temperature Measurement with Simple Compensation of Photo-Degradation Using Hydrogel-Tool and Color Space Conversion

Hisataka Maruyama; Taisuke Masuda; Fumihito Arai

doi:10.20965/jrm.2013.p0596

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JRM Vol.25 No.4 pp. 596-602

doi: 10.20965/jrm.2013.p0596

(2013)

Paper:

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Fluorescent-Based Temperature Measurement with Simple Compensation of Photo-Degradation Using Hydrogel-Tool and Color Space Conversion

Hisataka Maruyama, Taisuke Masuda, and Fumihito Arai

Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan

Received:

February 19, 2013

Accepted:

June 24, 2013

Published:

August 20, 2013

Keywords:

fluorescence, temperature sensor, color conversion, compensation, hydrogel-tool

Abstract

We developed a method to obtain stable and longlifetime temperature measurements using a fluorescence micromeasurement system. A hydrogel tool containing nano-semiconductor quantum dots (Q-dots) was developed as a fluorescent temperature indicator. We used image processing to convert RGB information to other color information to compensate for photodegradation. The temperature was calibrated using the hydrogel tool in several color spaces, including RGB (R: red, G: green, B: blue), HSV (H: hue, S: saturation, V: value (brightness)), and YCrCb (Y: brightness, Cr: red color difference, Cb: blue color difference). The calibration results showed that R, G, B, Y, and Cr decreased monotonically with increasing temperature, whereas H and Cb did not decrease monotonically. The photodegradation analysis showed that Cr was robust against the brightness fluctuation; however, R, G, and B strongly affected the brightness fluctuation because these values included the brightness information. These results show that temperature measurements based on Cr values are suitable to compensate for photodegradation and have a sensitivity of -1.3%/K and an accuracy of 0.3 K. These values are the same as those obtained using the fluorescence intensity method.

Cite this article as:

H. Maruyama, T. Masuda, and F. Arai, “Fluorescent-Based Temperature Measurement with Simple Compensation of Photo-Degradation Using Hydrogel-Tool and Color Space Conversion,” J. Robot. Mechatron., Vol.25 No.4, pp. 596-602, 2013.

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References

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[15] A. Al Salman, A. Tortschanoff, M. B. Mohamed, D. Tonti, F. van Mourik, and M. Cherguic, “Temperature effects on the spectral properties of colloidal CdSe nanodots, nanorods, and tetrapods,” Applied Physics Letters, Vol.90, pp. 093104-1-093104-3, 2007.
[16] H. Maruyama, T. Fukuda, and F. Arai, “Optical Adhesion Control of HydrogelMicrotools for On-Demand Immobilization andMeasurement of Cells on a Microfluidic Chip,” J. of Robotics and Mechatronics, Vol.22, No.5, pp. 631-638, 2010.
[17] W. Kunz, J. Henle, and B.W. Ninham, “‘Zur Lehre von derWirkung der Salze’ (about the science of the effect of salts): Franz Hofmeister’s historical papers,” Current Opinion in Colloid and Interface Science, Vol.9, pp. 19-37, 2004.
[18] H. Maruyama, T. Fukuda, and F. Arai, “Functional gel-microbead manipulated by optical tweezers for local environment measurement in mi-crochip,” Microfluidics and Nanofluidics, Vol.6, pp. 383-390, 2009.
[19] H. Stern and B. Efros, “Adaptive color space switching for tracking under varying illumination,” Image and Vision Computing, Vol.23, pp. 353-364, 2005.
[20] F. Arai, K. Yoshikawa, T. Sakami, and T. Fukuda, “Synchronized laser micromanipulation of multiple targets along each trajectory by single laser,” Applied Physics Letters, Vol.85, pp. 4301-4303, 2004.
[21] H. Maruyama, K. Kotani, T. Masuda, A. Honda, T. Takahata, and F. Arai, “Nanomanipulation of single influenza virus using dielectrophoretic concentration and optical tweezers for single virus infection to a specific cell on a microfluidic chip,” Microfluidics and Nanofluidics, Vol.10, pp. 1109-1117, 2011.
[22] M. Hagiwara, T. Kawahara, Y. Yamanishi, and F. Arai, “Precise Control of Magnetically Driven Microtools for Enucleation of Oocytes in a Microfluidic Chip,” Advanced Robotics, Vol.25, No.8, pp. 991-1005, 2011.

This article is published under a Creative Commons Attribution-NoDerivatives 4.0 Internationa License.

[1] [1] Y. Wakamoto, J. Ramsden, and K. Yasuda, “Single-cell growth and division dynamics showing epigenetic correlations,” Analyst, Vol.130, pp. 311-317, 2004.

[2] [2] N. Klauke, P.Monaghan et al., “Characterisation of spatial and temporal changes in pH gradients in microfluidic channels using optically trapped fluorescent sensors,” Lab on a Chip, Vol.6, pp. 788-793, 2006.

[3] [3] Z. Zhang and S. Achilefu, “Design, synthesis and evaluation of near-infrared fluorescent pH indicators in a physiologically relevant range,” Chem. Commun., pp. 5887-5889, 2005.

[4] [4] M. Suzuki, V. Tseeb, K. Oyama, and S. Ishiwata, “Microscopic detection of thermogenesisin a single HeLa cell,” Biophysical J., L46-L48, 2007.

[5] [5] T. Mirfakhrai, J. Oh, M. Kozlov, S. Fang, M. Zhang, R. Baughman, and J. Madden, “Mechanoelectrical Force Sensors Using Twisted Yarns of Carbon Nanotubes,” Trans. onMechatronics, Vol.16, No.1, pp. 90-97, 2011.

[6] [6] S. M. Borisov, C. Krause, S. Arain, and O. S. Wolfbeis, “CompositeMaterial for Simultaneous and Contactless Luminescent Sensing and Imaging of Oxygen and Carbon Dioxide,” Advanced Materials, Vol.18, pp. 1511-1516, 2006.

[7] [7] S. Nagl and O. S. Wolfbeis, “Optical multiple chemical sensing: status and current challenges,” The Analyst, Vol.132, pp. 507-511, 2007.

[8] [8] K. Sakaki, Z. Y. Shi, R. Kopelman, and H. Masuhara, “Three-Dimensional pH Microprobing with an Optically-Manipulated Fluorescent particle,” Chemistry Letters, Vol.25, pp. 141-142, 1996.

[9] [9] K. Onda and F. Arai, “Multi-beam bilateral teleoperation of holographic optical tweezers accelerated by general-purpose GPU,” Optics Express, Vol.20, pp. 3642-3653, 2012.

[10] [10] J. K. Cho, L. S.Wong et al., “PH Indicating resins,” Chemical Communications, pp. 1470-1471, 2004.

[11] [11] C.-H. Chang, H. Ogawa et al., “Healthcare chip based on integrated electrochemical sensors used for clinical diagnostics of BUN,” Japanese J. of Applied Physics, Vol.45, pp. 4241-4247, 2006.

[12] [12] H. Maruyama, F. Arai, and T. Fukuda, “On-Chip pH Measurement Using Functionalized Gel-Microbeads Positioned by Optical Tweezers,” Lab on a Chip, Vol.7, pp. 346-351, 2008.

[13] [13] J. Zhou, L. Li, Z. Gui, S. Buddhudu, and Y. Zhou, “Photoluminescence of CdSe nanocrystallites embedded in BaTiO3 matrix,” Applied Physics Letters, Vol.76, No.12, pp. 1540-1542, 2000.

[14] [14] G. W. Walker, V. C. Sundar, C. M. Rudzinski, A. W. Wun, M. G. Bawendi, and D. G. Nocera, “Quantum-dot optical temperature probes,” Applied Physics Letters, Vol.83, No.17, pp. 3555-3557, 2003.

[15] [15] A. Al Salman, A. Tortschanoff, M. B. Mohamed, D. Tonti, F. van Mourik, and M. Cherguic, “Temperature effects on the spectral properties of colloidal CdSe nanodots, nanorods, and tetrapods,” Applied Physics Letters, Vol.90, pp. 093104-1-093104-3, 2007.

[16] [16] H. Maruyama, T. Fukuda, and F. Arai, “Optical Adhesion Control of HydrogelMicrotools for On-Demand Immobilization andMeasurement of Cells on a Microfluidic Chip,” J. of Robotics and Mechatronics, Vol.22, No.5, pp. 631-638, 2010.

[17] [17] W. Kunz, J. Henle, and B.W. Ninham, “‘Zur Lehre von derWirkung der Salze’ (about the science of the effect of salts): Franz Hofmeister’s historical papers,” Current Opinion in Colloid and Interface Science, Vol.9, pp. 19-37, 2004.

[18] [18] H. Maruyama, T. Fukuda, and F. Arai, “Functional gel-microbead manipulated by optical tweezers for local environment measurement in mi-crochip,” Microfluidics and Nanofluidics, Vol.6, pp. 383-390, 2009.

[19] [19] H. Stern and B. Efros, “Adaptive color space switching for tracking under varying illumination,” Image and Vision Computing, Vol.23, pp. 353-364, 2005.

[20] [20] F. Arai, K. Yoshikawa, T. Sakami, and T. Fukuda, “Synchronized laser micromanipulation of multiple targets along each trajectory by single laser,” Applied Physics Letters, Vol.85, pp. 4301-4303, 2004.

[21] [21] H. Maruyama, K. Kotani, T. Masuda, A. Honda, T. Takahata, and F. Arai, “Nanomanipulation of single influenza virus using dielectrophoretic concentration and optical tweezers for single virus infection to a specific cell on a microfluidic chip,” Microfluidics and Nanofluidics, Vol.10, pp. 1109-1117, 2011.

[22] [22] M. Hagiwara, T. Kawahara, Y. Yamanishi, and F. Arai, “Precise Control of Magnetically Driven Microtools for Enucleation of Oocytes in a Microfluidic Chip,” Advanced Robotics, Vol.25, No.8, pp. 991-1005, 2011.