On-Demand and Size-Controlled Production of Droplets by Magnetically Driven Microtool
Lin Feng*, Tomohiro Kawahara*, Yoko Yamanishi*,
Masaya Hagiwara*, Kazuhiro Kosuge**,
and Fumihito Arai*
*Dept of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
**Tohoku University, 6-6-01 Aza-aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan
We have successfully produced size-controlled emulsion droplets on a chip by adjusting the vibration frequency for MMT. The novelty of this work is the fabrication of a thin coplanar Au electrode on the substrate of a microchip to work as a microsensor, and this microsensor contributed to a droplet-generation system with size estimation. When a droplet passes through the microsensor in the microchannel, it causes a change in the capacitance across a pair of microelectrodes in the microchannel, depending on the size of the droplet. We monitored the change in impedance in real time. The microsensor provided an output voltage proportional to the size of the droplet. The sensor output was observed by an oscilloscope at the primary stage. Manually we estimated the size and set a new actuation frequency for MMT to achieve on-demand and size control of the droplet. Real-time droplet detection was applied in this system. By monitoring the actuation frequency for MMT, size-controlled and ondemand droplet generation could be successfully carried out.
Masaya Hagiwara, Kazuhiro Kosuge, and
and Fumihito Arai, “On-Demand and Size-Controlled Production of Droplets by Magnetically Driven Microtool,” J. Robot. Mechatron., Vol.24, No.1, pp. 133-140, 2012.
-  C. Charcosset, I. Limayem, and H. Fessi, “The membrane emulsification process – a review,” J. of Chemical Technology and Biotechnology, Vol.79, pp. 209-218, 2004.
-  A. V. Korobko, W. Jesse, and J. R. C. Van der Maarel, “Encapsulation of DNA by Cationic Diblock Copolymer Vesicles,” Vol.21, pp. 34-42, 2005.
-  S. M. Moghimi, A. C. Hunter, and J. C. Murray, “Nanomedicine: current status and future prospects,” The FASEB Journal, Vol.19, pp. 311-330, 2005.
-  T. Kojima, Y. Takei, M. Ohtsuka, Y. Kawarasaki, T. Yamane, and H. Nakano, “PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets,” Nucleic Acids Research, Vol.33, Vol.17, e150, 2005.
-  C-W. Lai, Y-H. Lin, and G-B. Lee, “A microfluidic chip for formation and collection of emulsion droplets utilizing active pneumatic micro-choppers and micro-switches,” Biomedical Microdevices, Vol.10, pp. 749-756, 2008.
-  R. Heusch and B. A. G. Leverkusen, “Ullmann’s Encyclopedia of Industrial Chemistry,” 2000.
-  B. Zheng, J. D. Tice, and R. F. Ismagilov, “Formation of arrayed droplets by soft lithography and two-phase fluid flow, and application in protein crystallization,” Adv. Mater., Vol.16, No.15, pp. 1365-1368, 2004.
-  G. Yi, T. Thorsen, V. N. Manoharan, M. Hwang, S. Jeon, D. J. Pine, S. R. Quake, and S. Yang, “Generation of uniform colloidal assemblies in soft microfluidic devices,” Adv. Mater., Vol.15, No.15, pp. 1300-1304, 2003.
-  G. Yi, S. Jeon, T. Thorsen, V. N. Manoharan, S. R. Quake, D. J. Pine, and S. Yang, “Generation of uniform photonic balls by template-assisted colloidal crystallization,” Synth. Met. 139, pp. 803-806, 2003.
-  D. Dendukuri, K. Tsoi, T. A. Hatton, and P. S. Doyle, “Controlled synthesis of nonspherical microparticles using microfluidics,” Langmuir, Vol.21, pp. 2113-2116, 2005.
-  T. Nisisako, T. Torii, and T. Higuchi, “Novel microreactors for functional polymer beads,” Chem. Eng. J., Vol.101, pp. 23-29, 2004.
-  J. Lee and C. Kim, “Surface-tension-driven microactuation based on continuous electrowetting,” J. Microelectromech. Syst., Vol.9, No.2, pp. 171-180, 2000.
-  S.-K. Chae, C.-H. Lee, S. H. Lee, T.-.S Kim, and J. Y. Kang, “Oil droplet generation in PDMS microchannel using an amphiphilic continuous phase,” Lab Chip, Vol.9, pp. 1957-1961, 2009.
-  C.-H. Lee, S.-K. Hsiung, and G.-B. Lee, “An Active Flow Focusing Microfluidic Chip Utilizing Controllable Moving Walls for the Formation of Microdroplets in Liquid,” Proc. of the 2nd IEEE Int. Conf. on Nano/Micro Engineering and Molecular systems, Bangkok, Thailand, pp. 167-171, 2007.
-  T. Schneider, D. R. Burnham, J. VanOrden, and D. T. Chiu, “Systematic investigation of droplet generation at T-junctions,” T. Schneider, D. R. Burnham, J. VanOrden and D. T. Chiu (Eds.), Lab Chip, Vol.11, pp. 2055-2059, 2011.
-  S. Y. Park, T. H Wu, Y Chen, M. A. Teitell and P. Y Chiou, “Highspeed droplet generation on demand driven by pulse laser-induced cavitation”, Lab Chip, Vol.11, p. 1010-1012, 2011.
-  M. Hagiwara, T. Kawahara, Y. Yamanishi, and F. Arai, “Driving Method of Microtool by Horizontally-arranged Permanent Magnets for Single CellManipulation,” Applied Physics Letters, Vol.97, 013701, pp. 013701-1-013701-3, 2010.
-  Y. Yamanishi, S. Sakuma, K. Onda, and F. Arai, “Powerful Actuation of Magnetized Microtools by Focused Magnetic Field for Particle Sorting in a Chip,” Biomed Microdevices, Vol.12, pp. 745-752, 2010.
-  D. R. Link, S. L. Anna, D. A. Weitz, and H. A. Stone, “Geometrically Meditated Breakup of Drops in Microfluidic Devices,” Physical Review Letter, Vol.92, No.054503, 2004.
-  J. Z. Chen, A. A. Darhuber, S. M. Troian, and S. Wagner, “Capacitive sensing of droplets for microfluidic devices based on thermocapillary actuation,” Lab on a Chip. Jun; Vol.6, No.6, pp. 744-751, 2006.
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