Tooth Regenerative Therapy, Approached from Organogenesis
Kazuhisa Nakao*,** and Takashi Tsuji*,**
*Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
**Tissue Engineering Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
Regenerative medicine is expected to be a novel therapeutic system in this century [1-3]. The human body consists of 200 cell species generated from immature stem cells. In the 1990s, a treatment transplanting hematopoietic stem cells to replace all blood cells was established and successfully cured leukemia . With this as a model, stem cell transplantation therapy is being developed to restore the partial loss of organ function [5, 6]. The ultimate goal of regenerative medicine is to replace loss or damaged organs with artificial organs, so-called organ replacement therapy. Technical development to produce “tissues” made of a single cell species modeled on skin, bone, heart muscle, and cornea is advancing, but little development of organs per se has been attempted. In the sections that follow, we discuss why and explain how we are trying with the problems of “tooth regeneration.”
-  R. S. Langer and J. P. Vacanti, “Tissue engineering: the challenges ahead,” Sci. Am., 280, pp. 86-89, 1999.
-  J. P. Brockes and A. Kumar, “Appendage regeneration in adult vertebrates and implications for regenerative medicine,” Science, 310, pp. 1919-1923, 2005.
-  A. Atala, “Tissue engineering, stem cells and cloning: current concepts and changing trends,” Expert. Opin. Biol. Ther., 5, pp. 879-892, 2005.
-  F. R. Appelbaum, “Haematopoietic cell transplantation as immunotherapy,” Nature, 411, pp. 385-389, 2001.
-  J. H. Kim et al., “Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson’s disease,” Nature, 418, pp. 50-56, 2002.
-  L. C. Amado et al., “Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction,” Proc. Natl. Acad. Sci. USA, 102, pp. 11474-11479, 2005.
-  J. Pispa and I. Thesleff, “Mechanisms of ectodermal organogenesis,” Dev. Biol., 262, pp. 195-205, 2003.
-  I. Thesleff, “Epithelial-mesenchymal signalling regulating tooth morphogenesis,” J. Cell. Sci., 116, pp. 1647-1648, 2003.
-  A. Tucker and P. Sharpe, “The cutting-edge of mammalian development; how the embryo makes teeth,” Nat. Rev. Genet., 5, pp. 499-508, 2004.
-  P. T. Sharpe and C. S. Young, “Test-tube teeth,” Sci. Am., 293, pp. 34-41, 2005.
-  A. H. Yen and P. T. Sharpe, “Regeneration of teeth using stem cellbased tissue engineering,” Expert. Opin. Biol. Ther., 6, pp. 9-16, 2006.
-  S. Glasstone, “The Development of Tooth Germs in vitro,” J. Anat.,” 70, pp. 260-266, 3, 1936.
-  C. S. Young, S. Terada, J. P. Vacanti, M. Honda, J. D. Bartlett, and P. C. Yelick, “Tissue engineering of complex tooth structures on biodegradable polymer scaffolds,” J. Dent. Res., 81, pp. 695-700, 2002.
-  M. Honda, Y. Sumita, H. Kagami, and M. Ueda, “Histological and immunohistological studies of tissue engineered odontogenesis,” Arch. Histol. Cytol., 68, pp. 89-101, 2005.
-  Y. Sumita, M. J. Honda, T. Ohara, S. Tsuchiya, H. Sagara, H. Kagami, and M. Ueda, “Performance of collagen sponge as a 3-D scaffold for tooth-tissue engineering,” Biomaterials, 27, pp. 3238- 3248, 2006.
-  M. J. Honda, S. Tsuchiya, Y. Sumita, H. Sagara and M. Ueda, “The sequential seeding of epithelial and mesenchymal cells for tissue-engineering tooth regeneration,” Biomaterials, 4, pp. 680-689, 2007.
-  H. Yamamoto E.-J. Kim, S.-W. Cho, and H.-S. Jung, “Analysis of tooth formation by reaggregated dental mesenchyme form mouse embryo,” J. Electron Microsc. (Tokyo), 52, pp. 559-566, 2003.
-  B. Hu, A. Nadiri, S. Bopp-Kuchler, F. Perrin-Schmitt, S. Wang, and H. Lesot, “Dental epithelial histo-morphogenesis in the mouse: positional information versus cell history,” Arch. Oral. Biol., 50, pp. 131-136, 2005.
-  Y. Song et al., “Application of lentivirus-mediated RNAi in studying gene function in mammalian tooth development,” Dev. Dyn., 235, pp. 1334-1344, 2006.
-  B. Hu, A. Nadiri, S. Bopp-Kuchler, F. Perrin-Schmitt, H. Peters, and H. Lesot, “Tissue Engineering of Tooth Crown, Root, and Periodontium,” Tissue Eng., 8, pp. 2069-2075, 2006.
-  J. Yu, Y. Wang, Z. Deng, L. Tang, Y. Li, J. Shi, and Y. Jin, “Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells,” Biol. Cell, Epub ahead of print, 2007.
-  K. Nakao R. Morita, Y. Saji, K. Ishida, Y. Tomita, M. Ogawa, M. Saitoh, Y. Tomooka, and T. Tsuji, “The development of a bioengineered organ germ method,” Nat. Methods, 4, pp. 227-230, 2007.
-  A. Ohazama, S. A. C. Modino, I. Miletich, and P. T. Sharpe, “Stemcell-based tissue engineering of murine teeth,” J. Dent. Res., 83, pp. 518-522, 2004.
-  S. Gronthos, M. Mankani, J. Brahim, P. Gehron Robey, and S. Shi, “Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo,” Proc. Natl. Acad. Sci. USA, 97, pp. 13625-16330, 2000.
-  M. Miura, S. Gronthos, M. Zhao, B. Lu, L. W. Fisher, P. G. Robey, and S. Shi, “SHED: stem cells from human exfoliated deciduous teeth,” Proc. Natl. Acad. Sci. USA, 100, pp. 5807-5812, 2003.
-  B. M. Seo et al., “Investigation of multipotent postnatal stem cells from human periodontal ligament,” Lancet, 364, pp. 149-155, 2004.
-  W. Sonoyama et al., “Mesenchymal stem cell-mediated funtional tooth regeneration in Swine,” PLoS ONE, 1, e79, 2006.
-  A. Komine et al., “Tooth regeneration from newly established cell lines from a molar tooth germ epithelium,” Biochem. Biophys. Res. Commun., 355, pp. 758-763, 2007.
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