On-Chip Microparticle Manipulation Using Disposable Magnetically Driven Microdevices
Hisataka Maruyama*, Fumihito Arai**, and Toshio Fukuda*
*Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
**Department of Bioengineering and Robotics, Tohoku University, 6-6-01 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan
We propose novel microdevices that are driven by magnetic force for microparticles manipulation in a microchip. We developed two microdevices – a particle sorter and a particle filter. In the particle sorter, we perform sorting by switching the microchannel flow by driving a magnetic shuttle. In the particle filter, we perform size-based particle filtering by using the space between a magnetic shuttle and a microchannel. The microparticles which are bigger than the available space are stuck in the microchannels and solution passes through the space. These devices are easily implemented into a microchip at low cost. We make the microchip disposable by making the electromagnet part detachable. We succeeded in driving the magnetic shuttle in the microchannel and confirmed the functionality of the particle sorter and the particle filter. Our proposal has the following advantages: (1) Treatment of the target or complicated control systems are not needed, (2) Microdevices are easily implemented in a microchip, (3) The microchip is made disposable by making the electromagnet part detachable.
-  Y. Kimura, and R. Yanagimachi, “Intracytoplasmic sperm injection in the mouse,” Biology of Reproduction, Vol.52, No.4, pp. 709-720, 1995.
-  K. K. Tan, and S. C. Ng, “Computer-controlled piezo micromanipulation system for biomedical applications,” Engineering Science and Education Journal, pp. 249-256, 2001.
-  T. Nakayama, H. Fujiwara, K. Tatsumi, K. Fujita, T. Higuchi, and A. Sato, “A new assisted hatching technique using a piezomicromanipulator,” Fertility and Sterility, Vol.69, No.4, 1998.
-  M. R. Melamed, T. Lindmo, and M. L. Mendelsohn, “Flow Cytometry and Sorting,” Wiley-Liss, New York, USA, 2nd edn., 1991.
-  T. Katsuragi, and Y. Tani, “Single-Cell Sorting of Microorganisms by Flow or Slide-Based (Including Laser Scanning) Cytometry,” Acta Biotechnol., Vol.21, pp. 99-115, 2001.
-  S. Gawad, L. Schild, and Ph. Renaud, “Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing,” Lab on a chip, Vol.1, p. 76, 2001.
-  M. Rieseberg, C. Kasper, K. F. Reardon, and T. Scheper, “Flow Cytometry in Biotechnology,” Appl. Microbiol. Biotechnol., Vol.56, pp. 350-360, 2001.
-  H. E. Ayliffe, A. B. Frazier, and R. D. Rabbit, “Electric impedance spectroscopy using microchannels with integrated metal electrodes,” IEEE J. Microelectromech. Syst., Vol.8, No.1, p. 50, 1999.
-  S. Fiedler, S. G. Shirley, T. Schnelle, and G. Fuhr, “Dielectrophoretic Sorting of Particles and Cells in a Microsystem,” Anal. Chem., Vol.70, No.9, p. 1909, 1998.
-  C. K. Fuller, J. Hamilton, H. Ackler, and P. R. C. Gascoyne, “Microfabricated multi-frequency particle impedance characterization system,” Micro Total Analysis Systems, Kluwer, Enschede, Netherland, 2000.
-  G. B. Lee, C. I. Hung, B. J. Ke, G. R. Huang, B. H. Hwei, and H.-F. Lai, “Hydrodynamic Focusing for a Micromachined Flow Cytometer,” ASME J. Fluids Eng., Vol.123, pp. 672-679, 2001.
-  G.-B. Lee, L.-M. Fu, R.-J. Yang, and Y.-Y. Pan, “Micro Flow Cytometers Using Electrokinetic Forces with Integrated Optical Fibers for On-Line Cell/Particle Counting and Sorting,” 7th International Conference on Miniaturized Chemical and Biochemical Analysis Systems, Squaw Valley, California, USA, pp. 45-48, 2003.
-  J. Gao, X.-F. Yin, and Z.-L. Fang, “Integrating Single Cell Injection, Cell Lysis and Separation of Intracellular Constituents on a Microfluidic Chip,” 7th International Conference on Miniaturized Chemical and Biochemical Analysis Systems, Squaw Valley, California, USA, pp. 231-234, 2003.
-  E. B. Cummings, G. J. Fiechtner, A. K. Singh, B. A. Simmons, Y. Fintscenko, and B. Lapizco-Encinas, “Integrating Single Cell Injection, Cell Lysis and Separation of Intracellular Constitutions on a Microfluidic Chip,” 7th International Conference on Miniaturized Chemical and Biochemical Analysis Systems, Squaw Valley, California, USA, pp. 231-234, 2003.
-  T. Deng, G. M. Whitesides, M. Radhakrishnan, G. Zabow, and M. Prentiss, “Manipulation of magnetic microbeads in suspension using micromagnetic systems fabricated with soft lithography,” Applied Physics Letters, Vol.78, Issue 12, pp. 1775-1777, 2001.
-  H. Lee, A. M. Purdon, and R. M. Westervelt, “Manipulation of biological cells using a microelectromagnet matrix,” Applied Physics Letters, Vol.85, Issue 6, pp. 1603-1605, 2004.
-  N. A. Cridland, N. R. Sabour, and R. D. Saunders, “Effects of 50Hz magnetic field exposure on the rate of RNA synthesis by normal human fibroblasts,” International Journal of Radiation Biology, Vol.75, No.5, pp. 647-654, 1999.
-  M. Kuhara, H. Takeyama, T. Tanaka, and T. Matsunaga, “Magnetic Cell Separation Using Antibody Binding with Protein A Expressed on Bacterial Magnetic Particles,” Analytical Chemistry, Vol.76, No.21, pp. 6207-6213, 2004.
-  M. Gauthier, and E. Piat, “An electromagnetic micromanipulation system for single-cell manipulation,” Journal of Micromechatronics, Vol.2, No.2, p. 87, 2004.
-  E. Delamarche, A. Bernard, H. Schmid, B. Michel, and H. Biebucyck, “Patterned delivery of immunoglobulins to surface using microfluidic networks,” Science, Vol.276, p. 779, 1997.
-  C. S. Effenhauser, G. J. M. Brun, A. Paulus, and M. Ehrat, “Integrated capillary electrophoresis on flexible silicone microdevices: Analysis of DNA restriction fragments and detection of single DNA molecules on microchip,” Anal. Chem., 69, 3451, 1997.
-  J. M. K. Ng, I. Gitlin, A. D. Stroock, and G. M. Whitesides, “Components for integrated poly (dimethylsiloxane) microfluidic systems,” Electrophoresis, Vol.23, p. 3461, 2002.
-  K. Hosokawa, and R. Maeda, “A pneumatically-actuated three-way microvalve fabricated with poludimethylsiloxane using the membrane transfer technique,” J. Micromech. Meicroeng., Vol.10, p. 415, 2000.
This article is published under a Creative Commons Attribution-NoDerivatives 4.0 Internationa License.
Copyright© 2006 by Fuji Technology Press Ltd. and Japan Society of Mechanical Engineers. All right reserved.