The biomicromanipulation so important to biology and bioengineering remains difficult because object used are very small, kept in liquids, or observed by optical microscopy. We are developing a micromanipulation system for anatomical operation of microobjects such as embryos, cells, and microbes. Microscope images are 2-D, so it is difficult to manipulate targets in 3-D space. To improve manipulation work, we proposed a 3-D biomicromanipulation system combined with virtual reality (VR) space. Here we propose the 3-D modeling method of the object to present 3-D visual information to the operator and improve the operation environment. In this system, we still have difficulty changing the orientation of the microscopic object by manipulating a mechanical manipulator. The bioaligner we propose is a microdevice for the positional control of an object. We developed a 2-D bioaligner by microfabrication. Here we show its fabrication process and basic rotating experiment with chlorella cells.