Self-organizing maps are unsupervised neural network models that lend themselves to the cluster analysis of high-dimensional input data. Interpreting a trained map is difficult because features responsible for specific cluster assignment are not evident from resulting map representation. This paper presents an approach to automated knowledge acquisition using Kohonen’s self-organizing maps and k-means clustering. To demonstrate the architecture and validation, a data set representing animal world has been used as the training data set. The verification of the produced knowledge base is done by using conventional expert system.