A Puncturing Device that Mimics the Mechanism of Mosquito’s Proboscis and Labium - Verification of the Effect of Skin Deformation / Needle Buckling Prevention Mechanism and Puncture Experiment on Artificial Skin and Experimental Animals –

Shunki Yamamoto; Seiji Aoyagi; Masahiro Yamada; Tomokazu Takahashi; Masato Suzuki; Toshio Nagashima; Atsushi Kunugi; Makoto Chiyonobu; Takeshi Kuroiwa; Ryota Hosomi; Kenji Fukunaga; Daisuke Uta; Tomonori Takazawa; Tomoyuki Hikitsuchi; Yumi Kawajiri; Koji Nakayama

doi:10.20965/ijat.2020.p0117

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IJAT Vol.14 No.1 pp. 117-127

doi: 10.20965/ijat.2020.p0117

(2020)

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A Puncturing Device that Mimics the Mechanism of Mosquito’s Proboscis and Labium - Verification of the Effect of Skin Deformation / Needle Buckling Prevention Mechanism and Puncture Experiment on Artificial Skin and Experimental Animals –

Shunki Yamamoto^1,†, Seiji Aoyagi^1, Masahiro Yamada^1, Tomokazu Takahashi^1, Masato Suzuki^1, Toshio Nagashima^2, Atsushi Kunugi^3, Makoto Chiyonobu^3, Takeshi Kuroiwa^3, Ryota Hosomi^1, Kenji Fukunaga^1, Daisuke Uta^4, Tomonori Takazawa^5, Tomoyuki Hikitsuchi^6, Yumi Kawajiri^6, and Koji Nakayama^6

^*1Kansai University
3-3-35 Yamate-cho, Suita-shi, Osaka 564-8680, Japan

^†Corresponding author

^*2Sophia University, Tokyo, Japan

^*3JSOL Corporation, Tokyo, Japan

^*4University of Toyama, Toyama, Japan

^*5Gunma University Hospital, Maebashi, Japan

^*6Dainihon Jochugiku Co., Ltd., Osaka, Japan

Received:

July 9, 2019

Accepted:

August 23, 2019

Published:

January 5, 2020

Keywords:

microneedle, buckling, deformation, laser lithography, finite element method analysis

Abstract

This paper proposes a mechanism for preventing needle buckling and skin deformation by mimicking the mosquito’s labium and discusses a puncturing device with a jig-integrated microneedle, based on the proposed mechanism. A sheet simplifying this mechanism was attached to an artificial skin’s surface, and experiments to puncture this artificial skin and corresponding finite element method (FEM) analysis were conducted. It was confirmed that the deformation of the puncture target and the puncture resistance force decreased with the use of the sheet. Based on these experimental and FEM-analytical results, a puncturing device with a jig-integrated needle has been designed and fabricated with 3D laser lithography. Experiments have been conducted with the fabricated device to puncture an artificial skin and the skin of a nude mouse to determine needle buckling prevention and the reduction in skin deformation. The study successfully samples blood from the mouse without stagnation of blood flow.

Cite this article as:

S. Yamamoto, S. Aoyagi, M. Yamada, T. Takahashi, M. Suzuki, T. Nagashima, A. Kunugi, M. Chiyonobu, T. Kuroiwa, R. Hosomi, K. Fukunaga, D. Uta, T. Takazawa, T. Hikitsuchi, Y. Kawajiri, and K. Nakayama, “A Puncturing Device that Mimics the Mechanism of Mosquito’s Proboscis and Labium - Verification of the Effect of Skin Deformation / Needle Buckling Prevention Mechanism and Puncture Experiment on Artificial Skin and Experimental Animals –,” Int. J. Automation Technol., Vol.14 No.1, pp. 117-127, 2020.

Data files:

References

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This article is published under a Creative Commons Attribution-NoDerivatives 4.0 Internationa License.

[1] [1] S. Aoyagi, H. Izumi, and M. Fukuda, “Biodegradable Polymer Needle with Various Tip Angles and Consideration on Insertion Mechanism of Mosquito’s Proboscis,” Sensors & Actuators, Vol.A143/1, pp. 20-28, 2008.

[2] [2] H. Izumi and S. Aoyagi, “Novel Fabrication Method for Long Silicon Microneedles with Three-Dimensional Sharp Tips and Complicated Shank Shapes by Isotropic Dry Etching,” Trans. on Electrical and Electronic Engineering, Vol.2, No.3, pp. 328-334, 2007.

[3] [3] S. Aoyagi, “Visualization in Development of Microneedle – Development of Painless Needle Imitating Mosquito –,” J. of the Visualization Society of Japan, Vol.33, No.131, pp. 145-148, 2013 (in Japanese).

[4] [4] H. Kitada, H. Yamamoto, S. Yamamoto, M. Suzuki, S. Aoyagi, T. Takahashi, K. Fukunaga, R. Hosomi, T. Takazawa, D. Uta, Y. Kawajiri, K. Nakayama, and T. Hikitsuchi, “Observation of Mosquito Skin Penetration and Blood Suction in laboratory animals,” Proc. of 2017 JSPE Autumn Meeting, N-18, pp. 897-898, 2017 (in Japanese).

[5] [5] A. N. Clement, “The Biology of Mosquitoes,” CABI Publishing, pp. 224-234, 2000.

[6] [6] S. Aoyagi, “Overview of Microneedles,” J. of the Japan Society for Precision Engineering, Vol.82, No.12, pp. 999-1004, 2016 (in Japanese).

[7] [7] C.-H. Huang, T. Tanaka, Y. Takaoki, H. Izumi, T. Takahashi, M. Suzuki, and S. Aoyagi, “Fabrication of Metallic Microneedle by Electroplating and Sharpening of it by Electrochemical Etching,” The Trans. of the Institute of Electrical Engineers of Japan, Sec. E (Sensors and Micromachines), Vol.131, No.11, pp. 373-380, 2011 (in Japanese).

[8] [8] T. Tanaka, T. Takahashi, M. Suzuki, and S. Aoyagi, “Development of Minimally Invasive Microneedle Made of Tungsten – Sharpening Through Electrochemical Etching and Hole Processing for Drawing up Liquid Using Excimer Laser –,” J. Robot. Mechatron., Vol.25, No.4, pp. 755-761, 2013.

[9] [9] Y. Hara, M. Yamada, C. Tatsukawa, T. Takahashi, M. Suzuki, and S. Aoyagi, “Fabrication of Stainless Steel Microneedle with Laser-Cut Sharp Tip and its Penetration and Blood Sampling Performance,” Int. J. Automation Technol., Vol.10, No.6, pp. 950-957, 2016.

[10] [10] Y. Hara, M. Yamada, C. Tatsukawa, T. Takahashi, M. Suzuki, and S. Aoyagi, “Laser Fabrication of Jagged-Shaped Stainless Steel Microneedle Imitating Mosquito’s Maxilla,” Int. J. Automation Technol., Vol.10, No.6, pp. 958-964, 2016.

[11] [11] M. Suzuki, T. Sawa, T. Takahashi, and S. Aoyagi, “Fabrication of Microneedle Mimicking Mosquito Proboscis Using Nanoscale 3D Laser Lithography System,” Int. J. Automation Technol., Vol.9, No.6, pp. 655-661, 2015.

[12] [12] M. Suzuki, T. Sawa, Y. Terada, T. Takahashi, and S. Aoyagi, “Fabrication of Microneedles Precisely Imitating Mosquito’s Proboscis by Nanoscale Tree Dimensional Laser Lithography and Its Characterization,” The 18th Int. Conf. on Solid-State Sensors, Actuators and Microsystems (Transducers 2015), M4D.007, pp. 121-124, 2015.

[13] [13] S. Yamamoto, M. Suzuki, T. Takahashi, S. Aoyagi, T. Nagashima, S. Yamaguchi, S. Imazato, A. Kunugi, and T. Saruwatari, “Visualization of strain distribution and investigation on optimum condition in microneedle insertion,” Proc. of 2016 JSPE Autumn Meeting, C-21, pp. 103-104, 2016 (in Japanese).

[14] [14] Y. Urushibata, Y. Takaoki, T. Tanaka, T. Takahashi, M. Suzuki, S. Aoyagi, T. Kanzaki, S. Yamaguchi, and S. Imazato, “Explicit Finite Element Analysis of Mosquito’s Needle Insertion Mechanism,” Proc. of JSME Conf. on Robotics and Mechatronics (Robomec 2012), 1A1-R07, 2012 (in Japanese).

[15] [15] S. Yamamoto, M. Suzuki, T. Takahashi, S. Aoyagi, T. Nagashima, S. Yamaguchi, S. Imazato, A. Kunugi, and T. Saruwatari, “Confirmation of cooperative vibration effect of mosquito’s mouthpart by non-linear finite element method analysis,” Proc. of 2016 JSPE Spring Meeting, E-08, pp. 311-312, 2016 (in Japanese).

[16] [16] S. Yamamoto, T. Takahashi, M. Suzuki, S. Aoyagi, T. Nagashima, A. Kunugi, M. Chiyonobu, and T. Kuroiwa, “Puncture simulation of microneedle by nonlinear finite element method analysis – Examination of the effect of puncturing multiple needles and applying vibration at the time of needle puncturing –,” Proc. of 2019 JSPE Spring Meeting, D-37, pp. 347-348, 2019 (in Japanese).

[17] [17] Livermore Software Technology Corporation (LSTC), LS-DYNA official site. http://www.lstc.com/products/ls-dyna [Accessed July 2, 2019].

[18] [18] J. Donea, A. Huerta, J.-Ph. Ponthot, and A. Rodriguez-Ferran, “Arbitrary Lagrangian-Eulerian Methods,” E. Stein, R. Borst, and T. J. R. Hughes (Eds.), “Encyclopedia of Computational Mechanics,” John Wiley & Sons, Ltd., 2004.

[19] [19] S. Yamaguchi, K. Tsutsui, K. Satake, S. Morikawa, Y. Shirai, and H. T. Tanaka, “Dynamic analysis of a needle insertion for soft materials: Arbitrary Lagrangian-Eulerian-based three-dimensional finite element analysis,” Computers in Biology and Medicine, Vol.53, pp. 42-47, 2014.

[20] [20] D. Roylance, “Engineering viscoelasticity,” MIT web site. http://web.mit.edu/course/3/3.11/www/modules/visco.pdf [Accessed July 2, 2019].

[21] [21] Nanoscribe GmbH official site. https://www.nanoscribe.de/ [Accessed July 2, 2019].